Genomic and single-cell analyses reveal genetic signatures of swimming pattern and diapause strategy in jellyfish

• Published in: Nature communications Vol. 15; no. 1; pp. 5936 - 16
• Main Authors: Dong, Zhijun, Wang, Fanghan, Liu, Yali, Li, Yongxue, Yu, Haiyan, Peng, Saijun, Sun, Tingting, Qu, Meng, Sun, Ke, Wang, Lei, Ma, Yuanqing, Chen, Kai, Zhao, Jianmin, Lin, Qiang
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 15.07.2024; Nature Publishing Group UK; Nature Portfolio
• Subjects: Animals; Ciliary movement; Cnidaria; Developmental stages; Diapause; Diapause - genetics; Evolution; Evolutionary genetics; Gene Expression Profiling; Genes; Genetic analysis; Genetics; Genome - genetics; Genomes; Genomics - methods; Kinases; Laboratories; Locomotion; Morphogenesis; Pacemakers; Pattern analysis; Phosphorylation; Polyps (organisms); Positive selection; Proteins; Scyphozoa - genetics; Scyphozoa - physiology; Single-Cell Analysis; Statocysts; Swimming; Transcriptome; Transcriptomics; Turritopsis rubra
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-024-49848-z

Jellyfish exhibit innovative swimming patterns that contribute to exploring the origins of animal locomotion. However, the genetic and cellular basis of these patterns remains unclear. Herein, we generated chromosome-level genome assemblies of two jellyfish species, Turritopsis rubra and Aurelia coerulea, which exhibit straight and free-swimming patterns, respectively. We observe positive selection of numerous genes involved in statolith formation, hair cell ciliogenesis, ciliary motility, and motor neuron function. The lineage-specific absence of otolith morphogenesis- and ciliary movement-related genes in T. rubra may be associated with homeostatic structural statocyst loss and straight swimming pattern. Notably, single-cell transcriptomic analyses covering key developmental stages reveal the enrichment of diapause-related genes in the cyst during reverse development, suggesting that the sustained diapause state favours the development of new polyps under favourable conditions. This study highlights the complex relationship between genetics, locomotion patterns and survival strategies in jellyfish, thereby providing valuable insights into the evolutionary lineages of movement and adaptation in the animal kingdom.