Determination of thiabendazole residues in whole citrus fruits by liquid chromatography with fluorescence detection

• Published in: Journal of AOAC International Vol. 79; no. 2; p. 579
• Main Authors: Arenas, R.V. (Merck Research Laboratories, Three Bridges, NJ.), Rahman, H, Johnson, N.A
• Format: Journal Article
• Language: English
• Published: England 01.03.1996
• Subjects: ALIMENTOS; ANALISIS CUANTITATIVO; ANALYSE QUANTITATIVE; CHROMATOGRAPHIE; Chromatography, Ion Exchange; Chromatography, Liquid; CITRUS; Citrus - chemistry; CONTAMINACION; CONTAMINATION; CROMATOGRAFIA; FONGICIDE; FRUITS; FRUTAS; FUNGICIDAS; Fungicides, Industrial - analysis; MEDICION; MESURE; Pesticide Residues - analysis; PRODUIT ALIMENTAIRE; RESIDU; RESIDUOS; Spectrometry, Fluorescence; TECHNIQUE ANALYTIQUE; TECNICAS ANALITICAS; THIABENDAZOLE; Thiabendazole - analysis; TIABENDAZOL
• ISSN: 1060-3271; 1944-7922
• DOI: 10.1093/jaoac/79.2.579

An existing liquid chromatographic (LC) method for determination of thiabendazole (TBZ) residues in or on whole green bananas and potatoes was applied to whole, unwashed citrus fruits. The method is applicable for determining TBZ residues in whole oranges, grapefruits, tangerines, and lemons. TBZ is extracted from citrus homogenate with ethyl acetate, and the extract is cleaned up on a cation-exchange, solid-phase extraction column. The purified extract is analyzed by LC with a cation-exchange column and fluorescence detection. Average recovery of TBZ from whole citrus fruits fortified with TBZ at 0.05-20 ppm was 96%. The assay provides a simple, rapid, and sensitive method for monitoring TBZ residues in whole citrus fruits