Lytic KFS-SE2 phage as a novel bio-receptor for Salmonella Enteritidis detection

• Published in: The journal of microbiology Vol. 57; no. 2; pp. 170 - 179
• Main Authors: Choi, In Young, Lee, Cheonghoon, Song, Won Keun, Jang, Sung Jae, Park, Mi-Kyung
• Format: Journal Article
• Language: English
• Published: Seoul The Microbiological Society of Korea 01.02.2019; 한국미생물학회
• Subjects: Bacteriophages - classification; Bacteriophages - genetics; Bacteriophages - isolation & purification; Bacteriophages - ultrastructure; Base Composition; Biomedical and Life Sciences; Biosensing Techniques; DNA, Viral - genetics; Genes, Viral; Genome, Viral; Host Specificity; Life Sciences; Microbiology; Phylogeny; Salmonella enteritidis - isolation & purification; Salmonella enteritidis - virology; Sequence Analysis, DNA; Siphoviridae - classification; Siphoviridae - genetics; Viral Proteins - genetics; Virology; 생물학; Enteritidis; biosensor; lytic; bio-receptor; phage; Salmonella Enteritidis
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-019-8610-0

Since Salmonella Enteritidis is one of the major foodborne pathogens, on-site applicable rapid detection methods have been required for its control. The purpose of this study was to isolate and purify S. Enteritidis-specific phage (KFS-SE2 phage) from an eel farm and to investigate its feasibility as a novel, efficient, and reliable bio-receptor for its employment. KFS-SE2 phage was successfully isolated at a high concentration of (2.31 ± 0.43) × 10 11 PFU/ml, and consisted of an icosahedral head of 65.44 ± 10.08 nm with a non-contractile tail of 135.21 ± 12.41 nm. The morphological and phylogenetic analysis confirmed that it belongs to the Pis4avirus genus in the family of Siphoviridae . KFS-SE2 genome consisted of 48,608 bp with 45.7% of GC content. Genome analysis represented KFS-SE2 to have distinctive characteristics as a novel phage. Comparative analysis of KFS-SE2 phage with closely related strains confirmed its novelty by the presence of unique proteins. KFS-SE2 phage exhibited excellent specificity to S. Enteritidis and was stable under the temperature range of 4 to 50°C and pH of 3 to 11 ( P < 0.05). The latent time was determined to be 20 min. Overall, a new lytic KFS-SE2 phage was successfully isolated from the environment at a high concentration and the excellent feasibility of KFS-SE2 phage was demonstrated as a new bio-receptor for S. Enteritidis detection.