VIDAS enzyme-linked fluorescent immunoassay for detection of Salmonella in foods: collaborative study

• Published in: Journal of AOAC International Vol. 80; no. 3; p. 491
• Main Authors: Curiale, M.S. (Silliker Laboratories Group, Inc., South Holland, IL.), Gangar, V, Gravens, C
• Format: Journal Article
• Language: English
• Published: England 01.05.1997
• Subjects: ALIMENTOS; Animals; Bacteriological Techniques; Cacao - microbiology; CONTAMINACION; CONTAMINACION BIOLOGICA; CONTAMINATION; CONTAMINATION BIOLOGIQUE; Eggs - microbiology; Enzyme-Linked Immunosorbent Assay - methods; EVALUACION; EVALUATION; Fluoroimmunoassay - methods; Food Microbiology; Glycine max - microbiology; Milk - microbiology; PRODUIT ALIMENTAIRE; SALMONELLA; Salmonella - isolation & purification; TECHNIQUE IMMUNOLOGIQUE; TECNICAS INMUNOLOGICAS; Turkeys - microbiology
• ISSN: 1060-3271; 1944-7922
• DOI: 10.1093/jaoac/80.3.491

The VIDAS SLM method for detection of Salmonella was compared with the Bacteriological Analytical Manual (BAM)/AOAC culture method in a collaborative study. Twenty laboratories participated in the evaluation. Each laboratory tested one or more of 6 test products: milk chocolate, nonfat dry milk, dried whole egg, soy flour, ground black pepper, and ground raw turkey. No significant differences (P 0.05) were observed between the 2 methods. The 2 methods were in agreement for 99% of 1544 samples analyzed. Of the 20 samples out of agreement, 8 were VIDAS SLM positive and BAM/AOAC negative, and 12 were VIDAS SLM negative and BAM/AOAC positive. The VIDAS SLM method for detection of Salmonella in foods has been adopted first action by AOAC INTERNATIONAL