Early and rapid detection of UCHL1 in the serum of brain-trauma patients: a novel gold nanoparticle-based method for diagnosing the severity of brain injury

The clinical diagnosis of traumatic brain injury (TBI) is based on neurological examination and neuro-imaging tools such as CT scanning and MRI. However, neurological examination at times may be confounded by consumption of alcohol or drugs and neuroimaging facilities may not be available at all cen...

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Published inAnalyst (London) Vol. 143; no. 14; pp. 3366 - 3373
Main Authors Singh, Gyaninder Pal, Nigam, Richa, Tomar, Gaurav Singh, Monisha, Mohan, Bhoi, Sanjeev Kumar, S, Arulselvi, Sengar, Kangana, Akula, Deepa, Panta, Prashanth, Anindya, Roy
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 09.07.2018
Subjects
Biomarkers
Biomarkers - blood
Brain
Brain Injuries - blood
Brain Injuries - diagnosis
Computed tomography
Gold
Head injuries
Humans
Magnetic resonance imaging
Medical imaging
Metal Nanoparticles
Nanoparticles
Neurology
Patients
Sensitivity analysis
Traumatic brain injury
Ubiquitin Thiolesterase - blood
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Summary:The clinical diagnosis of traumatic brain injury (TBI) is based on neurological examination and neuro-imaging tools such as CT scanning and MRI. However, neurological examination at times may be confounded by consumption of alcohol or drugs and neuroimaging facilities may not be available at all centers. Human ubiquitin C-terminal hydrolase (UCHL1) is a well-accepted serum biomarker for severe TBI and can be used to detect the severity of a head injury. A reliable, rapid, cost effective, bedside and easy to perform method for the detection of UCHL1 is a pre-requisite for wide clinical applications of UCHL1 as a TBI biomarker. We developed a rapid detection method for UCHL1 using surface plasmon resonance of gold nanoparticles with a limit of detection (LOD) of 0.5 ng mL −1 . It has a sensitivity and specificity of 100% each and meets an analytical precision similar to that of conventional sandwich ELISA but can be performed rapidly. Using this method we successfull y detected UCHL1 in a cohort of 66 patients with TBI and were reliably able to distinguish mild TBI from moderate to severe TBI. We provide a gold nanoparticle-based method for traumatic brain injury diagnosis.
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ISSN:0003-2654
1364-5528
DOI:10.1039/c8an00533h