Superior sensitivity of conjugates of horseradish peroxidase with wheat germ agglutinin for studies of retrograde axonal transport

We have compared the retrograde axonal transport of horseradish peroxidase (HRP), to the retrograde transport of HRP conjugated with wheat germ agglutinin (WGA). Morphometric studies have shown that WGA-HRP conjugates were 40 times more sensitive than free HRP, in the tracing of retrograde connectio...

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Published inThe journal of histochemistry and cytochemistry Vol. 27; no. 3; pp. 728 - 734
Main Authors Gonatas, NK, Harper, C, Mizutani, T, Gonatas, JO
Format Journal Article
LanguageEnglish
Published Los Angeles, CA Histochemical Soc 01.03.1979
SAGE Publications
Subjects
Animals
Axonal Transport
Ganglia, Spinal - metabolism
Horseradish Peroxidase - metabolism
Hypoglossal Nerve - metabolism
Lectins - metabolism
Male
Peroxidases
Plant Lectins
Rats
Submandibular Gland - metabolism
Tongue - metabolism
Triticum
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Summary:We have compared the retrograde axonal transport of horseradish peroxidase (HRP), to the retrograde transport of HRP conjugated with wheat germ agglutinin (WGA). Morphometric studies have shown that WGA-HRP conjugates were 40 times more sensitive than free HRP, in the tracing of retrograde connections from the rat submandibular gland to the superior cervical ganglion. Also, WGA-HRP was more sensitive than free HRP in the tracing of retrograde connections from the rat tongue to the hypoglossal nucleus. Our findings with WGA-HRP are consistent with the observations by Schwab et al. who reported (-125I) WGA is a highly sensitive retrograde tracer (Brain Research 152:145, 1978 (22)).
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0022-1554
1551-5044
DOI:10.1177/27.3.90065