Detection of bixin, lycopene, canthaxanthin, and beta-apo-8'-carotenal in products derived from red pepper

• Published in: Journal of AOAC International Vol. 78; no. 2; p. 491
• Main Authors: Minguez-Mosquera, M.I. (Instituto de la Grasa y sus Derivados, Sevilla, Spain), Hornero-Mendez, D, Garrido-Fernandez, J
• Format: Journal Article
• Language: English
• Published: England 01.03.1995
• Subjects: ADULTERACION; ADULTERANTS; ADULTERATION; BIXA ORELLANA; Canthaxanthin - analysis; Capsicum; CAPSICUM ANNUUM; CAROTENOIDE; CAROTENOIDES; CAROTENOIDS; Carotenoids - analysis; CHROMATOGRAPHIE; CHROMATOGRAPHIE EN COUCHE MINCE; CHROMATOGRAPHY; Chromatography, Liquid - methods; Chromatography, Thin Layer - methods; CROMATOGRAFIA; CROMATOGRAFIA DE CAPA FINA; Food Coloring Agents - chemistry; FOOD PASTES; FORMULACIONES; FORMULATION; FORMULATIONS; LIQUID CHROMATOGRAPHY; Lycopene; OLEORESINAS; OLEORESINE; OLEORESINS; PAPRIKA; PAPRIKA PASTE; PIMENTON; Plants, Medicinal; THIN LAYER CHROMATOGRAPHY; TOMATE; TOMATOES
• ISSN: 1060-3271; 1944-7922
• DOI: 10.1093/jaoac/78.2.491

An analytical method using either thin layer or liquid chromatography is proposed for the detection of 4 pigments (bixin, lycopene, canthaxanthin and beta-apo-8'-carotenal) that can be used fraudulently to intensify the natural color of products derived from red pepper (oleoresins, paprika, paprika paste, etc.). Similarly, the addition of other colorant natural products containing some of these pigments as major pigments (such as tomato for lycopene and Bixa orellana seeds for bixin) can be detected. The method proposed can also be used to control the aforementioned pigments in their natural sources as well as in food products.