In situ formation of biphasic calcium phosphates and their biological performance in vivo
The co-precipitation technique has been applied to synthesize biphasic calcium phosphate (BCP). After annealing at 900°C for 24h, hydroxyapatite (HAp) and β-tricalcium phosphate (β-TCP) were obtained as a single phase at 1.67 and 1.5 Ca/P ratios, respectively. Between these two extremes, a whole ran...
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Published in | Ceramics international Vol. 38; no. 3; pp. 1965 - 1974 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Ltd
01.04.2012
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Subjects | |
Online Access | Get full text |
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Summary: | The co-precipitation technique has been applied to synthesize biphasic calcium phosphate (BCP). After annealing at 900°C for 24h, hydroxyapatite (HAp) and β-tricalcium phosphate (β-TCP) were obtained as a single phase at 1.67 and 1.5 Ca/P ratios, respectively. Between these two extremes, a whole range of BCP preparations could be synthesized by using this technique with an accurate control of starting reactants. The biological performance of BCP granulates with a specific content of 62% HAp and 38% β-TCP was investigated. After immersion in Hanks’ balanced salt solution (HBSS) for 1 week, a precipitation started to be formed with individual small granules on the specimen surface. An MTT assay indicated that BCP granulates have no cytotoxic effects on MG-63 cells, and that they have good biocompatibility. An implantation experiment in mouse skulls revealed that BCP granulate provides a strong positive effect on bone formation in vivo in mice. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0272-8842 1873-3956 |
DOI: | 10.1016/j.ceramint.2011.10.029 |