A polysaccharide of Dendrobium officinale ameliorates H2O2-induced apoptosis in H9c2 cardiomyocytes via PI3K/AKT and MAPK pathways

• Published in: International journal of biological macromolecules Vol. 104; no. Pt A; pp. 1 - 10
• Main Authors: Zhang, Jing-yi, Guo, Ying, Si, Jin-ping, Sun, Xiao-bo, Sun, Gui-bo, Liu, Jing-jing
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2017
• Subjects: active ingredients; antioxidant activity; Apoptosis; cardiomyocytes; cardioprotective effect; cardiovascular diseases; Dendrobium officinale; hydrogen peroxide; lipid peroxidation; MAPK; membrane potential; mitochondrial membrane; mitogen-activated protein kinase; Oriental traditional medicine; Oxidative stress; PI3K/Akt; Polysaccharide; polysaccharides; signal transduction; survival rate; Oxidative stress; Dendrobium officinale; MAPK; Polysaccharide; Apoptosis; PI3K/Akt
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2017.05.169

•In this paper, we isolated and purified a homogeneous polysaccharide with molecular weight of 951.9kDa composed of glucose:galactose:mannose=2.13:1.34:1.00.•D. officinale polysaccharide may protest H9c2 cardiomyocytes apoptosis from H2O2-induced oxidative damage.•D. officinale polysaccharide could be applicable to therapeutics for myocardial ischemia/reperfusion injuries. Dendrobium officinale is one valuable traditional Chinese medicine, which has skyscraping medicinal value. Polysaccharide is the main active ingredient in D. officinale; its antioxidant activity is a hot research topic nowadays. Oxidative stress plays an important role in the pathological progress of a variety of cardiovascular disease, as one of key factors of cardiomyocyte apoptosis. This research adopts a model of H2O2 induction-H9c2 cardiomyocytes apoptosis, aiming to study the effect of Dendrobium officinale Polysaccharide (DOP-GY) for cardiomyocyte apoptosis caused by oxidative stress and its possible mechanism. Our results showed that pretreatment of DOP-GY (low dose: 6.25μg/mL, medium dose: 12.5μg/mL, high dose: 25μg/mL) followed by a 2h incubation with 200μM H2O2 elevated the survival rate, cutted the LDH leakage, reduced lipid peroxidation damage, improved the activity of the endogenous antioxidant enzymes. In addition, the pretreatment of DOP-GY significantly inhibited the production of ROS, declined of the mitochondrial membrane potential, down-regulated pro-apoptosis protein and up-regulated anti-apoptosis protein. The protective effect was correlated with the PI3K/Akt and MAPK signal pathway. Collectively, these observations suggest that DOY-GY has the potential to exert cardioprotective effects against H2O2-induced H9c2 cardiomyocyte apoptosis.