Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa

• Published in: Archives of pharmacal research Vol. 35; no. 12; pp. 2135 - 2142
• Main Authors: Van Kiem, Phan, Van Minh, Chau, Nhiem, Nguyen Xuan, Cuong, Nguyen Xuan, Tai, Bui Huu, Quang, Tran Hong, Anh, Hoang Le Tuan, Yen, Pham Hai, Ban, Ninh Khac, Kim, Seung Hyun, Xin, Mingjie, Cha, Ji-Yun, Lee, Young-Mi, Kim, Young Ho
• Format: Journal Article
• Language: English
• Published: Heidelberg Pharmaceutical Society of Korea 01.12.2012; 대한약학회
• Subjects: Anti-Inflammatory Agents, Non-Steroidal - isolation & purification; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Cell Survival - drug effects; Cell Survival - physiology; Dose-Response Relationship, Drug; Ficus; Glycolipids - isolation & purification; Glycolipids - physiology; HT29 Cells; Humans; Interleukin-8 - antagonists & inhibitors; Interleukin-8 - secretion; Medicine; Pharmacology/Toxicology; Pharmacy; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plant Leaves - chemistry; Plant Leaves - physiology; Research Article; Tumor Necrosis Factor-alpha - antagonists & inhibitors; Tumor Necrosis Factor-alpha - physiology; 약학; Inflammation; HT-29 cell line; Moraceae; IL-8; Galactolipid
• ISSN: 0253-6269; 1976-3786
• DOI: 10.1007/s12272-012-1210-8

Bioassay-guided fractionation based on the anti-inflammatory activity of a methanol extract of Ficus microcarpa leaves led to the isolation of seven galactolipids: 2( S )-3- O -octadeca-9 Z ,12 Z ,15 Z -trienoylglyceryl- O -β- D -galactopyranoside ( 1 ), (2 S )-2,3- O -dioctadeca-9 Z ,12 Z ,15 Z -trienoylglyceryl- O -β- D -galactopyranoside ( 2 ), (2 S )-2,3- O -dioctadeca-9 Z ,12 Z -dienoylglyceryl- O -β- D -galactopyranoside ( 3 ), (2 S )-3- O -octadeca-9 Z ,12 Z ,15 Z -trienoylglyceryl-6′- O -(α- D -galactopyranosyl)-β- D -galactopyranoside ( 4 ), (2 S )-2,3- O -dioctadeca-9 Z ,12 Z ,15 Z -trienoylglyceryl-6’- O -(α-D-galactopyranosyl)-β-D-galactopyranoside ( 5 ), gingerglycolipid B ( 6 ), and (2 S )-2,3- O -dioctadeca-9 Z ,12 Z -dienoylglyceryl-6′- O -(α- D -galactopyranosyl)-β-D-galactopyranoside ( 7 ). Their chemical structures were elucidated by mass, 1D-, and 2D-NMR spectroscopic methods as well as chemical methods. The antiinflammatory effect of these compounds on TNF-α induced IL-8 secretion in the HT-29 cell line was evaluated. All above galactolipids showed significant inhibition ranging 40% at a concentration of 50 μM. The results suggest that galactolipids from the leaves of F. microcarpa may be used as potent anti-inflammatory agents.