Single-cell, locus-specific bisulfite sequencing (SLBS) for direct detection of epimutations in DNA methylation patterns

Stochastic epigenetic changes drive biological processes, such as development, aging and disease. Yet, epigenetic information is typically collected from millions of cells, thereby precluding a more precise understanding of cell-to-cell variability and the pathogenic history of epimutations. Here we...

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Bibliographic Details
Published inNucleic acids research Vol. 43; no. 14; p. e93
Main Authors Gravina, Silvia, Ganapathi, Shireen, Vijg, Jan
Format Journal Article
LanguageEnglish
Published England Oxford University Press 18.08.2015
Subjects
Animals
Cells, Cultured
DNA Methylation
Epigenesis, Genetic
Genetic Loci
Methods Online
Mice
Mice, Inbred C57BL
Mutation Rate
Promoter Regions, Genetic
Sequence Analysis, DNA - methods
Single-Cell Analysis
Sulfites
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Summary:Stochastic epigenetic changes drive biological processes, such as development, aging and disease. Yet, epigenetic information is typically collected from millions of cells, thereby precluding a more precise understanding of cell-to-cell variability and the pathogenic history of epimutations. Here we present a novel procedure for directly detecting epimutations in DNA methylation patterns using single-cell, locus-specific bisulfite sequencing (SLBS). We show that within gene promoter regions of mouse hepatocytes the epimutation rate is two orders of magnitude higher than the mutation rate.
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ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkv366