Synthesis, characterization, antimicrobial and antibiofilm activity, and molecular docking analysis of NHC precursors and their Ag-NHC complexes

• Published in: Dalton transactions : an international journal of inorganic chemistry Vol. 5; no. 42; pp. 154 - 15412
• Main Authors: Üstün, Elvan, ahin, Neslihan, Çelik, Cem, Tutar, U ur, Özdemir, Nam k, Gürbüz, Nevin, Özdemir, smail
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 02.11.2021
• Subjects: Anti-Bacterial Agents - chemical synthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Anti-Infective Agents - chemical synthesis; Anti-Infective Agents - chemistry; Anti-Infective Agents - pharmacology; Antibiotics; Antiinfectives and antibacterials; Benzimidazoles - chemical synthesis; Benzimidazoles - chemistry; Benzimidazoles - pharmacology; Biofilms; Biofilms - drug effects; Chemical analysis; Chemical synthesis; Chloride; Chlorides; Coordination Complexes - chemical synthesis; Coordination Complexes - chemistry; Coordination Complexes - pharmacology; Crystallography; Heterocyclic Compounds - chemistry; Heterocyclic Compounds - pharmacology; Infrared analysis; Infrared spectroscopy; Methane - analogs & derivatives; Methane - chemistry; Methane - pharmacology; Microbial Sensitivity Tests; Microorganisms; Molecular docking; Molecular Docking Simulation; Molecular Structure; NMR spectroscopy; Precursors; Silver - chemistry; Silver - pharmacology; Single crystals; Spectrum analysis
• ISSN: 1477-9226; 1477-9234; 1477-9234
• DOI: 10.1039/d1dt02003j

Microorganisms attach to surfaces and interfaces and form biofilms which create a sheltered area for host cell response. Therefore, biofilms provide troubles in fields such as medicine, food, and pharmaceuticals. Inhibition of formation of biofilms through hindering of quorum sensing could be a method for the production of new generation antibiotics. In this study, four new benzimidazole type NHC precursors (1-allyl-3-benzyl-5,6-dimethylbenzimidazolium chloride, 1-allyl-3-(2,4,6-trimethylbenzyl)-5,6-dimethylbenzimidazolium chloride, 1-allyl-3-(2,3,5,6-tetramethylbenzyl)-5,6-dimethylbenzimidazolium chloride, and 1-allyl-3-(2,3,4,5,6-pentamethylbenzyl)-5,6-dimethylbenzimidazolium chloride and Ag-NHC complexes of these molecules were synthesized and characterized by elemental analysis, FT-IR spectroscopy, 1 H, and 13 C{ 1 H} NMR spectroscopy, LC-MS, and single crystal crystallography. Antimicrobial and biofilm formation inhibition activities of the molecules were evaluated. In addition, the activities of the molecules were examined in detail by molecular docking analysis. According to the results obtained, higher activity was achieved with the complex molecules when compared with the benzimidazole derivative ligands. A series of novel silver( i )-NHC complexes are synthesized and characterized. The anticandidal activity of some complexes was found to be similar to that of Flucanozol.