Direct radioimmunoassay of active and inactive human glandular kallikrein: some physiological and pathological variabilities

We have developed a sensitive and specific radioimmunoassay which allows the detection of human glandular kallikrein in biologic fluids at a level of 40 pg/ml. The antisera did not recognize human plasma kallikrein and glandular kallikrein from other species including marmoset. Furthermore the antib...

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Published inJournal of immunoassay (Monticello, N.Y.) Vol. 10; no. 2-3; p. 221
Main Authors Girolami, J P, Pecher, C, Bascands, J L, Cabos-Boutot, G, Vega-Vidalle, C, Colle, A, Adam, A, Suc, J M
Format Journal Article
LanguageEnglish
Published United States 01.06.1989
Subjects
Adolescent
Adult
Aged
Animals
Antibody Specificity
Child
Child, Preschool
Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
Female
Humans
Kallikreins - analysis
Kidney Transplantation
Lymph - analysis
Male
Middle Aged
Postoperative Period
Rabbits
Radioimmunoassay
Time Factors
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Summary:We have developed a sensitive and specific radioimmunoassay which allows the detection of human glandular kallikrein in biologic fluids at a level of 40 pg/ml. The antisera did not recognize human plasma kallikrein and glandular kallikrein from other species including marmoset. Furthermore the antibody did not bind pro-kallikrein but was specific for the trypsin activated kallikrein. The antibody inhibited the kininogenase activity of standard kallikrein incubated with human kininogen. However active kallikrein inhibited by inhibitors bound at the active site is still detectable, indicating that the antibody is specific for the structure of the active form but not for the active site. In normotensive subjects, daily urinary kallikrein excretion increased with age until 30, then a decrease was observed. In renal transplanted recipients a progressive increase of the active form was found. A low concentration of immunoreactive active kallikrein was detected in lymphatic fluids of patients suffering from acute pancreatitis treated by lymphatic drainage; although this kallikrein is the active immunoreactive form, a very weak kininogenase activity was measured, suggesting a partial inhibition by anti-proteases. These data provide complementary evidence for the physiological and pathological role of glandular kallikrein.
ISSN:0197-1522
DOI:10.1080/01971528908053238