Stacked traits conferring multiple resistance to imazamox and glufosinate in soft wheat

• Published in: Pest management science Vol. 75; no. 3; pp. 648 - 657
• Main Authors: Domínguez‐Mendez, Rafael, Alcántara‐de la Cruz, Ricardo, Rojano‐Delgado, Antonia M, da Silveira, Hellen Martins, Portugal, João, Cruz‐Hipolito, Hugo Enrique, De Prado, Rafael
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.03.2019; Wiley Subscription Services, Inc
• Subjects: Acetolactate Synthase; Agricultural production; Aminobutyrates - metabolism; Aminobutyrates - pharmacology; Ammonia; Carbon 14; Cultivars; Glufosinate; Glutamate-Ammonia Ligase; Glutamine; glutamine synthase; Herbicide resistance; Herbicide Resistance - genetics; herbicide resistance crops; Herbicides; Herbicides - pharmacology; Imidazoles - metabolism; Imidazoles - pharmacology; Mutation; N‐acetyl‐glufosinate; Organic chemistry; Parents; Phosphinothricin; phosphinothricin acetyl transferase; Translocation; Triticum - enzymology; Triticum - genetics; Triticum aestivum; Weed control; Wheat; phosphinothricin acetyl transferase; N-acetyl-glufosinate; herbicide resistance crops; acetolactate synthase; Triticum aestivum; glutamine synthase
• ISSN: 1526-498X; 1526-4998
• DOI: 10.1002/ps.5159

BACKGROUND Conventional crossing of soft wheat cultivars resistant to imazamox and glufosinate resulted in two (Rados and Helter) lines resistant to both herbicides. Stacked traits conferring this dual herbicide resistance in these lines, compared with a susceptible (S) cultivar, were characterized. RESULTS Rados and Helter lines were ∼ 18‐fold more resistant (R) to glufosinate, and between 15.1 and 19.8‐fold more resistant to imazamox than the S cultivar. Resistance to glufosinate and imazamox decreased up to 12% and 50%, respectively, when the herbicides were applied sequentially. The basal activities of the acetolactate and glutamine synthases were similar between R and S plants. Rados and Helter lines were 11.7‐ and 17.7‐fold more resistant to imazamox than the S cultivar, due to the Ser653–Asn mutation in their imi‐ALS genes. R lines, susceptible to glufosinate at the target site level, showed lower ammonia accumulation evidencing the activity of the phosphinothricin acetyl transferase. Absorption and translocation patterns for 14C‐imazamox and 14C‐glufosinate were similar between R and S cultivars and so do not contribute to resistance. CONCLUSION Stacked traits conferring dual herbicide resistance to the lines Rados and Helter come from the resistant parents. These R lines are potential tools for weed management in wheat production, mainly via herbicide rotation. © 2018 Society of Chemical Industry Crossing soft wheat cultivars resistant to imazamox and glufosinate resulted in two lines with dual herbicide resistance. These lines are potential tools for weed management in wheat production via herbicide rotation.