Toward a zero waste approach: Utilization of sugarcane bagasse for dye removal and multienzymes production

• Published in: Journal of basic microbiology Vol. 64; no. 2; pp. e2300529 - n/a
• Main Authors: Ejaz, Uroosa, Taj, Ayaz, Sohail, Muhammad, Alanazi, Abdullah K., Abo‐dief, Hala M.
• Format: Journal Article
• Language: English
• Published: Germany 01.02.2024
• Subjects: adsorbents; amylases; azo dyes; Brevibacillus borstelensis; carbon; cell walls; endo-1,4-beta-glucanase; energy; fermentation; fungi; laccase; lignin; malachite green; Neobacillus sedimentimangrovi; plant cell wall degrading enzymes; polygalacturonase; polysaccharides; sugarcane bagasse; sugars; textile mill effluents; Trametes pubescens; xylanases; zero wastes; Brevibacillus borstelensis; malachite green; Neobacillus sedimentimangrovi; Trametes pubescens; plant cell wall degrading enzymes
• ISSN: 0233-111X; 1521-4028; 1521-4028
• DOI: 10.1002/jobm.202300529

Global production of sugarcane bagasse (SB) by sugar industries exceeds more than 100 tons per annum. SB is rich in lignin and polysaccharide and hence can serve as a low‐cost energy and carbon source for the growth of industrially important microorganism. However, various other applications of SB have also been investigated. In this study, SB was used as an adsorbent to remove an azo dye, malachite green. Subsequently, the dye‐adsorbed SB was fermented by Trametes pubescens MB 89 for the production of laccase enzyme. The fungal pretreated SB was further utilized as a substrate for the simultaneous production of multiple plant cell wall degrading enzymes including, cellulase, xylanase, pectinase, and amylase by thermophilic bacterial strains. Results showed that 0.1% SB removed 97.04% malachite green at 30°C after 30 min from a solution containing 66 ppm of the dye. Fermentation of the dye‐adsorbed SB by T. pubescens MB 89 yielded 667.203 IU mL−1 laccase. Moreover, Brevibacillus borstelensis UE10 produced 38.41 and 18.6 IU mL−1 β‐glucosidase and pectinase, respectively, by using fungal‐pretreated SB. Cultivation of B. borstelensis UE27 in the medium containing the same substrate yielded 32.14 IU mL−1 of endoglucanase and 27.23 IU mL−1 of β‐glucosidase. Likewise, Neobacillus sedimentimangrovi UE25 could produce a mix of β‐glucosidase (37.24 IU mL−1), xylanase (18.65 IU mL−1) and endoglucanase (26.65 IU mL−1). Hence, this study led to the development of a method through which dye‐containing textile effluent can be treated by SB along with the production of industrially important enzymes.