Selected ion monitoring gas chromatography/mass spectrometry using uniformly labelled (13C)glucose for determination of glucose turnover in man

An optimized method is described for the mass fragmentographic determination of uniformly labelled (13C)glucose in human plasma using a butylboronic acid acetate derivative, and capillary gas chromatography. The advantages of the method are the ease and speed of the derivatization procedure, the sma...

Full description

Saved in:
Bibliographic Details
Published inBiological mass spectrometry Vol. 20; no. 4; p. 203
Main Authors Pickert, A, Overkamp, D, Renn, W, Liebich, H, Eggstein, M
Format Journal Article
LanguageEnglish
Published England 01.04.1991
Subjects
Adult
Blood Glucose - metabolism
Carbon Isotopes
Gas Chromatography-Mass Spectrometry - methods
Humans
Male
Online AccessGet more information

Cover

More Information
Summary:An optimized method is described for the mass fragmentographic determination of uniformly labelled (13C)glucose in human plasma using a butylboronic acid acetate derivative, and capillary gas chromatography. The advantages of the method are the ease and speed of the derivatization procedure, the small sample size, high precision (interassay coefficient of variation 5.7%), and applicability of a relatively low-cost mass spectrometer. This method allows glucose tracer experiments to be performed in man using the bolus injection technique, necessitating analysis of many samples. The results on glucose turnover obtained in a clinical experiment were in full agreement with previously published data.
ISSN:1052-9306
DOI:10.1002/bms.1200200408