Chip-based nanoflow high performance liquid chromatography coupled to mass spectrometry for profiling of soybean flavonoids

• Published in: Electrophoresis Vol. 33; no. 15; pp. 2399 - 2406
• Main Authors: Chang, Yuwei, Zhao, Chunxia, Wu, Zeming, Zhou, Jia, Zhao, Sumin, Lu, Xin, Xu, Guowang
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.08.2012
• Subjects: Chromatography, High Pressure Liquid - methods; Flavonoids; Flavonoids - analysis; Flavonoids - isolation & purification; Glycine max - chemistry; Glycine max - classification; HPLC-chip/MS; Least-Squares Analysis; Linear Models; Metabolic profiling; Microchip Analytical Procedures - methods; Reproducibility of Results; Sensitivity and Specificity; Soybean; Tandem Mass Spectrometry - methods
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/elps.201100581

In this work a chip‐based nano HPLC coupled MS (HPLC‐chip/MS) method with a simple sample preparation procedure was developed for the flavonoid profiling of soybean. The analytical properties of the method including the linearity (R2, 0.992–0.995), reproducibility (RSD, 1.50–7.66%), intraday precision (RSD, 1.41–5.14%) and interday precision (RSD, 2.76–16.90%) were satisfactory. Compared with the conventional HPLC/MS method, a fast extraction and analysis procedure was applied and more flavonoids were detected in a single run. Additionally, 13 flavonoids in soybean seed were identified for the first time. The method was then applied to the profiling of six varieties of soybean sowed at the same place. A clear discrimination was observed among different cultivars, three isoflavones, accounting for nearly 80% of total flavonoid contents, were found increased in the spring soybeans compared with the summer cultivars.