Evolution of multiple target‐site resistance mechanisms in individual plants of glyphosate‐resistant Eleusine indica from China

• Published in: Pest management science Vol. 77; no. 10; pp. 4810 - 4817
• Main Authors: Zhang, Chun, Yu, Chao‐Jie, Yu, Qin, Guo, Wen‐Lei, Zhang, Tai‐Jie, Tian, Xing‐Shan
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.10.2021; Wiley Subscription Services, Inc
• Subjects: (CT)n insertion; 3-Phosphoshikimate 1-Carboxyvinyltransferase - genetics; 3-Phosphoshikimate 1-Carboxyvinyltransferase - metabolism; 5' Untranslated Regions; Copy number; copy number variation (CNV); DNA Copy Number Variations; Eleusine - genetics; Eleusine - metabolism; Eleusine indica; EPSPS gene mutation; EPSPS gene overexpression; Evolution; Gene Expression Regulation, Plant; Glycine - analogs & derivatives; Glyphosate; glyphosate resistance; Herbicide resistance; Herbicide Resistance - genetics; Herbicides - pharmacology; Homology; Insertion; Low level; Mutation; Populations; Progeny; Weed control; China; copy number variation (CNV); (CT)n insertion; EPSPS gene overexpression; EPSPS gene mutation; glyphosate resistance; Eleusine indica
• ISSN: 1526-498X; 1526-4998
• DOI: 10.1002/ps.6527

BACKGROUND Glyphosate has been used for weed control in South China in various situations for four decades, and most Eleusine indica populations are suspected to have evolved resistance to glyphosate. This research investigated underling target‐site glyphosate resistance mechanisms in six field‐collected, putative glyphosate‐resistant (R) E. indica populations. RESULTS The six R E. indica populations were confirmed to be low (1.8 to 2.6‐fold) to moderately (5.6‐ to 8.4‐fold) resistant to glyphosate relative to the susceptible (S) population. Sixty‐seven glyphosate‐surviving plants from the six R populations were used to examine target‐site resistance mechanisms. Target‐site 5‐enolpyruvylshikimate3‐phosphate synthase (EPSPS) overexpression (OE) (plus further induction by glyphosate treatment) and gene copy number variation (CNV) occurred in 94% R plants, and among them, 16% had the P106A mutation and 49% had the heterozygous double TIPS (T102I + P106S) mutation (plus P381L). In addition, a low number of R plants (6%) only had the homologous TIPS (plus P381L) mutation. The (CT)6 insertion mutation in the EPSPS 5†‐UTR always associates with EPSPS OE and CNV. Progeny plants possessing EPSPS OE/CNV (and P106A) displayed low level (up to 4.5‐fold) glyphosate resistance. In contrast, plants homozygous for the TIPS mutation displayed higher (25‐fold) resistance to glyphosate and followed by plants heterozygous for this mutation plus EPSPS OE/CNV (12‐fold). CONCLUSIONS Target‐site glyphosate resistance in E. indica populations from South China is common with prevalence of EPSPS OE/induction/CNV conferring low level resistance. Individual plants acquiring both the TIPS mutation and EPSPS OE/CNV are favored due to evolutionary advantages. The role of (CT)6 insertion mutation in EPSPS CNV is worth further investigation. © 2021 Society of Chemical Industry. This study showcased the adaptability of Eleusine indica plants to abiotic stress (herbicide) by evolving EPSPS gene mutations (especially the TIPS double mutation), EPSPS induction, overexpression, and copy number variation.