Spheroids and organoids derived from colorectal cancer as tools for in vitro drug screening

Colorectal cancer (CRC) is a heterogeneous disease. Conventional two-dimensional (2D) culture employing cell lines was developed to study the molecular properties of CRC . Although these cell lines which are isolated from the tumor niche in which cancer develop, the translation to human model such a...

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Bibliographic Details
Published inExploration of targeted anti-tumor therapy Vol. 5; no. 2; pp. 409 - 431
Main Authors Ahmad Zawawi, Sahira Syamimi, Salleh, Elyn Amiela, Musa, Marahaini
Format Journal Article
LanguageEnglish
Published United States Open Exploration Publishing 2024
Open Exploration Publishing Inc
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Summary:Colorectal cancer (CRC) is a heterogeneous disease. Conventional two-dimensional (2D) culture employing cell lines was developed to study the molecular properties of CRC . Although these cell lines which are isolated from the tumor niche in which cancer develop, the translation to human model such as studying drug response is often hindered by the inability of cell lines to recapture original tumor features and the lack of heterogeneous clinical tumors represented by this 2D model, differed from condition. These limitations which may be overcome by utilizing three-dimensional (3D) culture consisting of spheroids and organoids. Over the past decade, great advancements have been made in optimizing culture method to establish spheroids and organoids of solid tumors including of CRC for multiple purposes including drug screening and establishing personalized medicine. These structures have been proven to be versatile and robust models to study CRC progression and deciphering its heterogeneity. This review will describe on advances in 3D culture technology and the application as well as the challenges of CRC-derived spheroids and organoids as a mode to screen for anticancer drugs.
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ISSN:2692-3114
2692-3114
DOI:10.37349/etat.2024.00226