TLC characterization of liposomes containing D-myo-inositol derivatives

• Published in: Biomedical chromatography Vol. 9; no. 4; p. 175
• Main Authors: Brailoiu, E, Huhurez, G, Slatineanu, S, Filipeanu, C M, Costuleanu, M, Branisteanu, D D
• Format: Journal Article
• Language: English
• Published: England 01.07.1995
• Subjects: Animals; Aorta - drug effects; Aorta - physiology; Chromatography, Thin Layer; Drug Carriers; Inositol 1,4,5-Trisphosphate - analysis; Inositol 1,4,5-Trisphosphate - chemistry; Inositol Phosphates - analysis; Inositol Phosphates - chemistry; Liposomes - chemistry; Male; Phosphatidylcholines - chemistry; Phytic Acid - administration & dosage; Phytic Acid - chemistry; Phytic Acid - pharmacology; Rats
• ISSN: 0269-3879
• DOI: 10.1002/bmc.1130090405

The thin-layer chromatographic (TLC) behaviour of liposomes containing inositol phosphates (IPs) was studied. The liposomes contained different concentrations of D-myo-inositol 1,4,5k-trisphosphate (IP3), D-myo-inositol 1,2,6-trisphosphate (alpha-trinositol, PP 56, a novel Perstorp Pharma derivative), D-myo-inositol 1,3,4,5-tetrakisphosphate (IP4), D-myo-inositol 1,3,4,5,6-pentakisphosphate (IP5) and D-myo-inositol 1,2,3,4,5,6-hexakisphosphate (IP6). Migration of all liposome batches was compared to that of control liposomes (containing only triple distilled water), and to that of free phosphatidylcholine (PC); the same amount of lipid was used in all situations. Thin-layer chromatography was performed on silica gel as adsorbent. As solvent we used an n-buthanol:ethanol:water mixture in a 4:3:3 volume ratio. Significant differences were found between PC and all liposome batches, as well as between control liposomes and the ones containing IP3, alpha-trinositol, IP4, or IP5, in various concentrations. Liposomes containing IP6 migrate completely differently compared not only to phosphatidylcholine and control liposomes, but also to the ones containing other IPs ( < 10(-3) M). Unlike the other IPs studied, liposome-entrapped IP6 elicits dose-dependent contractions of the isolated rat aorta. This suggests that liposomes loaded with IP6 undergo, during or after their preparation, physico-chemical alterations that eventually change their drug-delivery capacity.