An improved silver stain for the visualization of lipopolysaccharides on polyacrylamide gels

A sensitive, brief, and user‐friendly silver stain to meet the needs in high‐efficiency detection of lipopolysaccharides (LPS) on polyacrylamide gels is described. In this study, the most commonly used formaldehyde‐based LPS silver stain, which is potentially hazardous to the operator, is replaced b...

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Bibliographic Details
Published inElectrophoresis Vol. 33; no. 7; pp. 1220 - 1223
Main Authors Zhu, Zhong-Xin, Cong, Wei-Tao, Ni, Mao-Wei, Wang, Xi, Ma, Wei-De, Ye, Wei-Jian, Jin, Li-Tai, Li, Xiao-Kun
Format Journal Article
LanguageEnglish
Published Germany Blackwell Publishing Ltd 01.04.2012
Subjects
Ascorbic acid
Ascorbic Acid - chemistry
Electrophoresis, Polyacrylamide Gel - methods
Limit of Detection
Lipopolysaccharides
Lipopolysaccharides - analysis
Silver stain
Silver Staining - methods
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Summary:A sensitive, brief, and user‐friendly silver stain to meet the needs in high‐efficiency detection of lipopolysaccharides (LPS) on polyacrylamide gels is described. In this study, the most commonly used formaldehyde‐based LPS silver stain, which is potentially hazardous to the operator, is replaced by ascorbic acid (Vc) in alkaline sodium thiosulfate solution. It takes only about 35 min to complete all the protocol, with a detection limit of 4 ng of total LPS. The results indicate that this user‐friendly method could be a good choice for LPS visualization on polyacrylamide gels.
Bibliography:ark:/67375/WNG-F7MKGGGT-G
ArticleID:ELPS4165
istex:54108352D8E08E4EC63C637D6094D216DDD54985
These authors contributed equally to this work.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201100492