Development of an integrated optical analyzer for characterization of growth dynamics of bacterial colonies

In order to understand the biophysics behind collective behavior of a bacterial colony, a confocal displacement meter was used to measure the profiles of the bacterial colonies, together with a custom built optical density circuits. The system delivered essential information related to the quantitat...

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Published inJournal of biophotonics Vol. 6; no. 11-12; pp. 929 - 937
Main Authors Kim, Huisung, Bai, Nan, Bhunia, Arun K., King, Galen B., Hirleman, E. Daniel, Bae, Euiwon
Format Journal Article
LanguageEnglish
Published Berlin WILEY-VCH Verlag 01.12.2013
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
Subjects
bacterial colony morphology
diffraction
Escherichia coli O157 - growth & development
instrumentation and measurement
Lasers
light scattering
Optical Devices
Staphylococcus aureus - growth & development
Time Factors
light scattering
diffraction
bacterial colony morphology
instrumentation and measurement
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Summary:In order to understand the biophysics behind collective behavior of a bacterial colony, a confocal displacement meter was used to measure the profiles of the bacterial colonies, together with a custom built optical density circuits. The system delivered essential information related to the quantitative growth dynamics (height, diameter, aspect ratio, optical density) of the bacterial colony. For example, the aspect ratio of S. aureus was approximately two times higher than that of E. coli O157 : H7, while the OD of S. aureus was approximately 1/3 higher than that of E. coli O157 : H7. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Bibliography:istex:7823E99ABBDCF29E37761059DD700B1D50A3494C
cooperative agreement with the Agricultural Research Service of the US Department of Agriculture project number 1935-42000-072-02G and the Center for Food Safety and Engineering at Purdue University
ArticleID:JBIO201200224
ark:/67375/WNG-CCQCF0J1-4
ISSN:1864-063X
1864-0648
DOI:10.1002/jbio.201200224