The application of electrospray and neutral-loss mass spectrometry to the identification of the metabolites of S12813 in rat liver slices

The metabolism of S12813, (3-(2-[4-phenyl piperazin-1-yl] ethyl)-2-oxo-2,3-dihydro oxazolo [4,5-b] pyridine chlorohydrate), in rat liver slice incubates was examined by high performance liquid chromatography combined with mass spectrometry. Electrospray ionization was used together with tandem mass...

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Bibliographic Details
Published inRapid communications in mass spectrometry Vol. 8; no. 5; p. 361
Main Authors Brownsill, R, Combal, J P, Taylor, A, Bertrand, M, Luijten, W, Walther, B
Format Journal Article
LanguageEnglish
Published England 01.05.1994
Subjects
Analgesics - pharmacokinetics
Animals
Biotransformation
Chromatography, Liquid
Glucuronates - metabolism
In Vitro Techniques
Liver - metabolism
Male
Mass Spectrometry
Oxazoles - pharmacokinetics
Rats
Rats, Wistar
Sulfates - metabolism
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Summary:The metabolism of S12813, (3-(2-[4-phenyl piperazin-1-yl] ethyl)-2-oxo-2,3-dihydro oxazolo [4,5-b] pyridine chlorohydrate), in rat liver slice incubates was examined by high performance liquid chromatography combined with mass spectrometry. Electrospray ionization was used together with tandem mass spectrometric techniques of analysis (MS/MS). Polar phase I and phase II metabolites were identified as C-oxidation products, which were then conjugated to form either sulphate or glucuronide metabolites. On the basis of the identifications made, a metabolic pathway of S12813 in rat liver slices has been proposed.
ISSN:0951-4198
DOI:10.1002/rcm.1290080504