Quantitative gas chromatographic mass spectrometric determination of mandelic acid in blood plasma. Comparison of deuterated and homologous internal standards

Quantitative gas chromatography/mass spectrometry/selected ion monitoring of mandelic acid in plasma in the lower micromolar range has been investigated using both the deuterated compound and a homologue, 2-phenyllactic acid, as internal standards. On chromatography of the TMSi-ester ethers, the lat...

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Bibliographic Details
Published inBiomedical mass spectrometry Vol. 10; no. 3; p. 183
Main Authors Luthe, H, Ludwig-Köhn, H, Langenbeck, U
Format Journal Article
LanguageEnglish
Published England 01.03.1983
Subjects
Adult
Biological Transport
Deuterium
Gas Chromatography-Mass Spectrometry
Humans
Mandelic Acids - blood
Stereoisomerism
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Summary:Quantitative gas chromatography/mass spectrometry/selected ion monitoring of mandelic acid in plasma in the lower micromolar range has been investigated using both the deuterated compound and a homologue, 2-phenyllactic acid, as internal standards. On chromatography of the TMSi-ester ethers, the latter is less stable than the former. The chromatographic isotope effect observed for deuterated mandelic acid does not suggest a carrier function for this compound. Normal plasma levels of mandelic acid are about 0.5 microM. Only a minor portion of phenyramidol is metabolized to mandelic acid. Preliminary in vivo data indicate the presence of a stereoselective transport system for D(-)-mandelic acid in gastrointestinal tract and possibly kidney.
ISSN:0306-042X
DOI:10.1002/bms.1200100313