Chloroplast DNA variation of Tilia cordata (Tiliaceae)

• Published in: Canadian journal of forest research Vol. 33; no. 12; pp. 2503 - 2508
• Main Authors: Fineschi, S, Salvini, D, Taurchini, D, Carnevale, S, Vendramin, G G
• Format: Journal Article
• Language: English
• Published: Ottawa, Canada NRC Research Press 01.12.2003; National Research Council of Canada; Canadian Science Publishing NRC Research Press
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Classical and quantitative genetics. Population genetics. Molecular genetics; Deoxyribonucleic acid; DNA; Flora; Flowers & plants; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; Genetic diversity; Genetic resources, diversity; Genetics; Genetics and breeding of economic plants; Genetics of eukaryotes. Biological and molecular evolution; Haplotypes; Holocene; Molecular genetics; Plant material; Plant species; Pleistocene; Pollen; Population genetics; Population genetics, reproduction patterns; Pteridophyta, spermatophyta; Quaternary; Refugia; Species extinction; Tilia cordata; Trees; Vegetals; Europe; Genetic variability; Horticulture; Genomics; Chloroplast DNA; Arboriculture; Variations; Broadleaved ornamental tree; Genotype; Population structure; Genetic diversity; Molecular marker; Population genetics; Tilia cordata; Polymerase chain reaction; Restriction fragment length polymorphism; Dicotyledones; Tiliaceae; Angiospermae; Forestry; Hardwood forest tree; Spermatophyta; Molecular biology; Genome; Haplotype
• ISSN: 0045-5067; 1208-6037
• DOI: 10.1139/x03-179

The distribution of haplotypic diversity of 17 Tilia cordata Mill. populations was investigated by PCR–RFLP markers of the chloroplast genomes. A high number of haplotypes (14) and high total genetic diversity (h T = 0.881) were detected. The distribution of the chloroplast DNA haplotypes revealed low geographic structure of the genetic diversity; the coefficient of differentiation between populations, G st = 0.552, was lower than the mean value reported for maternally inherited markers in Angiosperm tree species. The value of population subdivision for ordered alleles, as measured by N st , was significantly higher than the value of population subdivision for unordered alleles (N st = 0.662, G st = 0.552), thus indicating the presence of a phylogeographic structure. The relatively low genetic differentiation among T. cordata populations may be explained mainly as a consequence of human impact on this species.