Expression analysis of protease-activated receptor-2 in cats

• Published in: Veterinary immunology and immunopathology Vol. 229; p. 110115
• Main Authors: Iio, Aki, Kaji, Kenjiro, Kaji, Noriyuki, Hori, Masatoshi, Yonezawa, Tomohiro, Momoi, Yasuyuki, Maeda, Shingo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2020
• Subjects: Animals; Cat; Cat Diseases - genetics; Cat Diseases - metabolism; Cats; Cell Line; Chronic kidney disease; CRFK; DNA, Complementary; HEK293 Cells; Humans; PAR-2; Receptor, PAR-2 - biosynthesis; Receptor, PAR-2 - genetics; Receptor, PAR-2 - metabolism; Renal Insufficiency, Chronic - genetics; Renal Insufficiency, Chronic - metabolism; Renal Insufficiency, Chronic - veterinary; Sequence Analysis, DNA; Tissue Distribution; Transcriptome; Trypsin - metabolism; PAR-2; Cat; Chronic kidney disease; HPRT; CRFK; RPL17; YWHAZ
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/j.vetimm.2020.110115

•Nucleotide sequence of feline protease-activated receptor-2 (PAR-2) gene was determined.•PAR-2 mRNA and protein was differentially expressed in different tissues of healthy cats.•Functional PAR-2 was expressed in Crandell-Rees Feline Kidney (CRFK) cells. Chronic kidney disease (CKD) is a common disease in geriatric cats. Despite its high prevalence, the pathogenesis of feline CKD is poorly understood. Recently, there has been increasing evidence for the role of protease-activated receptor-2 (PAR-2) in the progression of CKD in humans and rodents. However, the role of PAR-2 in feline CKD has not been evaluated. In this study, we determined nucleotide sequence of feline PAR-2 from the kidney, evaluated PAR-2 mRNA and protein expression in normal feline tissues, and analyzed functional expression in the feline kidney epithelial cell line Crandell-Rees Feline Kidney (CRFK). The open reading frame of feline PAR-2 comprised 1,194 bp and encoded 397 amino acids, showing 90%, 90%, and 85% identities to human, dog, and mouse PAR-2, respectively. In healthy cats, expression levels of the PAR-2 mRNA and protein were relatively higher in the gastrointestinal tract and kidney, and was lowest in the heart. The feline PAR-2 protein expression was confirmed, and stimulation of trypsin and PAR-2 agonists induced a prompt increase in the intracellular calcium ion concentration in CRFK cells. The present study will provide fundamental information for investigation of the involvement of PAR-2 in the pathogenesis of CKD in cats.