Enantioselective oxidative stress and oxidative damage caused by Rac- and S-metolachlor to Scenedesmus obliquus

• Published in: Chemosphere (Oxford) Vol. 173; pp. 22 - 30
• Main Authors: Liu, Huijun, Xia, YiLu, Cai, Weidan, Zhang, Yina, Zhang, Xiaoqiang, Du, Shaoting
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.04.2017
• Subjects: Acetamides - chemistry; Acetamides - pharmacology; Antioxidant enzyme; Catalase - metabolism; Cell ultrastructures; Cellular membrane permeability; Chlorophyll - metabolism; Chlorophyll content; Enantioselectivity; Herbicides - chemistry; Herbicides - pharmacology; Oxidative Stress - drug effects; Reactive Oxygen Species - metabolism; ROS; Scenedesmus - drug effects; Scenedesmus - growth & development; Scenedesmus - metabolism; Stereoisomerism; Superoxide Dismutase - metabolism; Water Pollutants, Chemical - pharmacology; NBT; FDA; Enantioselectivity; Chlorophyll content; DCF; Chl b; Cell ultrastructures; SOD; Chl a; APX; GST; Racmt; H2DCFDA; GR; Antioxidant enzyme; LSCM; H2DCF; Rac; ROS; CAT; Cellular membrane permeability; Smt; TEM
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2017.01.028

The rational use and environmental security of chiral pesticides has gained the interest of many researchers. The enantioselective effects of Rac- and S-metolachlor on oxidative stress in Scenedesmus obliquus were determined in this study. Stronger green fluorescence was observed in response to S-metolachlor treatment than to Rac-metolachlor treatment, suggesting that more reactive oxygen species (ROS) were stimulated by S-metolachlor. ROS levels following S-metolachlor treatment were 1.92-, 8.31-, and 1.08-times higher than those observed following Rac-metolachlor treatment at 0.1, 0.2, and 0.3 mg/L, respectively. Superoxide dismutase (SOD) and catalase (CAT) were stimulated with increasing herbicide concentrations, with S-metolachlor exhibiting a greater effect. Oxidative damage in terms of chlorophyll (Chl) content, cellular membrane permeability, and cellular ultrastructures of S. obliquus were investigated. Chla and Chlb contents in algae treated with Rac-metolachlor were 2–6-fold higher than those in algae treated with S-metolachlor at 0.1, 0.2, and 0.3 mg/L. The cellular membrane permeability of algae exposed to 0.3 mg/L Rac- and S-metolachlor was 6.19- and 42.5-times that of the control. Correlation analysis implied that ROS are the major factor responsible for the oxidative damage caused by Rac- and S-metolachlor. Damage to the chloroplasts and cell membrane of S. obliquus, low production of starch granules, and an increased number of vacuoles were observed upon ultrastructural morphology analysis by transmission electron microscope. These results indicate that S-metolachlor has a greater effect on S. obliquus than Rac-metolachlor. •ROS, SOD, and CAT were stimulated by Rac- and S-metolachlor in S. obliquus.•Oxidative damage affected chlorophyll, membrane permeability, and cell structures.•ROS promote oxidative damage caused by Rac- and S-metolachlor.•The effect of S-metolachlor was greater than that of Rac-metolachlor with enantioselectivity.