Genome-Wide Identification of Seven in Absentia E3 Ubiquitin Ligase Gene Family and Expression Profiles in Response to Different Hormones in Uncaria rhynchophylla

• Published in: International journal of molecular sciences Vol. 25; no. 14; p. 7636
• Main Authors: Lan, Jinxu, Lian, Conglong, Shao, Yingying, Chen, Suiqing, Lu, Ying, Zhu, Lina, Mu, Detian, Tang, Qi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.07.2024
• Subjects: Abscisic acid; Amino acids; bioinformatics; Biosynthesis; Chromosomes; Chromosomes, Plant - genetics; Enzymes; expression pattern; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes; Genome, Plant; Genomes; Genomics; Hormones; Hypertension; Ligases; Medicine, Botanic; Medicine, Herbal; Metabolism; Multigene Family; Phylogenetics; Phylogeny; Plant growth; Plant Growth Regulators - metabolism; Plant Growth Regulators - pharmacology; plant hormones; Plant metabolites; Plant Proteins - genetics; Plant Proteins - metabolism; Proteins; Senescence; Signal transduction; SINA; Stress, Physiological - genetics; Synteny; TIAs; Ubiquitin; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism; Uncaria rhynchophylla; China; TIAs; expression pattern; Uncaria rhynchophylla; bioinformatics; plant hormones; SINA
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25147636

SINA (Seven in absentia) E3 ubiquitin ligases are a family of RING (really interesting new gene) E3 ubiquitin ligases, and they play a crucial role in regulating plant growth and development, hormone response, and abiotic and biotic stress. However, there is little research on the SINA gene family in . In this study, a total of 10 genes were identified from the genome. The results of multiple sequence alignments and chromosomal locations show that 10 genes were unevenly located on 22 chromosomes, and each UrSINA protein contained a SINA domain at the N-terminal and RING domains at the C-terminal. Synteny analysis showed that there are no tandem duplication gene pairs and there are four segmental gene pairs in , contributing to the expansion of the gene family. Furthermore, almost all genes contained the same gene structure, with three exons and two introns, and there were many cis-acting elements relating to plant hormones, light responses, and biotic and abiotic stress. The results of qRT-PCR show that most genes were expressed in stems, with the least expression in roots; meanwhile, most genes and key enzyme genes were responsive to ABA and MeJA hormones with overlapping but different expression patterns. Co-expression analysis showed that UrSINA1 might participate in the TIA pathway under ABA treatment, and UrSINA5 and UrSINA6 might participate in the TIA pathway under MeJA treatment. The mining of genes in the provided novel information for understanding the gene and its function in plant secondary metabolites, growth, and development.