Characterization of simple sequence repeats in Japanese chestnut

Summary Fifteen SSR (simple sequence repeat) loci were developed in Japanese chestnut (Castanea crenata Sieb et Zucc.) using a genomic library enriched for (AG)/(TC). The SSR loci obtained produced 1-16 alleles per locus, of which 14 showed polymorphisms. The average values of heterozygosity and pol...

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Bibliographic Details
Published inThe journal of horticultural science & biotechnology Vol. 78; no. 2; pp. 197 - 203
Main Authors Yamamoto, T., Tanaka, T., Kotobuki, K., Matsuta, N., Suzuki, M., Hayashi, T.
Format Journal Article
LanguageEnglish
Published Taylor & Francis 2003
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Summary:Summary Fifteen SSR (simple sequence repeat) loci were developed in Japanese chestnut (Castanea crenata Sieb et Zucc.) using a genomic library enriched for (AG)/(TC). The SSR loci obtained produced 1-16 alleles per locus, of which 14 showed polymorphisms. The average values of heterozygosity and polymorphic information content among the 14 loci were 0.50 and 0.54, respectively. Genetic relatedness was evaluated for 30 Japanese chestnut accessions using 14 polymorphic SSR loci, with no distinct differences in allele composition observed between cultivated and wild natures as well as between Japanese and Korean origins. SSR loci developed in the Japanese chestnut could be successfully transferable to other Castanea species. Diversity and genetic relationship in Castanea pp. was examined based on the nucleotide sequences of putative SSR alleles.
ISSN:1462-0316
2380-4084
DOI:10.1080/14620316.2003.11511606