Plasma and urine DNA levels are related to microscopic hematuria in patients with bladder urothelial carcinoma

• Published in: Clinical biochemistry Vol. 49; no. 16-17; pp. 1274 - 1277
• Main Authors: de Almeida, Eduardo Ferreira Pedroso, Abdalla, Tomás Elias, Arrym, Tiago Pedromonico, de Oliveira Delgado, Pamela, Wroclawski, Marcelo Langer, da Costa Aguiar Alves, Beatriz, de S. Gehrke, Flávia, Azzalis, Ligia Ajaime, Alves, Sarah, Tobias-Machado, Marcos, de Lima Pompeo, Antonio Carlos, Fonseca, Fernando Luiz Affonso
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2016
• Subjects: Aged; Bladder cancer; DNA - blood; DNA - metabolism; DNA - urine; Female; Hematuria; Hematuria - blood; Hematuria - metabolism; Hematuria - urine; Humans; Male; Plasma DNA; Prognosis; Urinary Bladder Neoplasms - blood; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - urine; Urine DNA; Prognosis; Urine DNA; Hematuria; Bladder cancer; Plasma DNA
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/j.clinbiochem.2016.08.021

a) Objective: An increase in cell-free DNA was observed in the plasma of many cancer patients. This major biomarker can be used to differentiate patients with malignant neoplasms from those with benign neoplasms or healthy patients. Depending on the characteristic of the tumor, there are qualitative variations in the circulating cell-free DNA. Today, studies on the concentration of fragments of circulating cell-free DNA and their respective sizes in patients with bladder cancer are not plentiful in the literature. A 100% effective plasma tumor marker, which would help in the diagnosis and follow-up of bladder cancer, is yet to be developed; therefore, cell-free DNA levels in the plasma may represent a valuable biomarker for the diagnosis, prognosis and follow-up of patients with this type of tumor. b) Design and methods: In this study we analyze the kinetics of plasma and urine DNA concentrations in patients with bladder cancer, relating them to the other clinical laboratory variables. c) Results: Patients with hematuria showed a positive correlation with urine DNA. d) Conclusion: An increase in plasma and urine DNA was unprecedentedly reported over time, a fact that may come in handy in the prognosis of patients. Furthermore, microscopic haematuria is correlated with plasma and urinary DNA levels. •Hematuria related to plasma and urine DNA levels in bladder carcinoma•Bladder carcinoma patients and plasma and urine DNA levels•Hematuria is associated with DNA levels in plasma and urine samples.