Dual-labeled time-resolved immunofluorometric assay for the determination of IgM antibodies to rubella virus and cytomegalovirus in human serum
This study established a novel time-resolved fluorescence immunoassay (TRFIA) that allows the simultaneous determination of rubella virus (RV) IgM and cytomegalovirus (CMV) IgM in human serum. Lanthanum elements labeled antibody and streptavidin–biotin system were used in the “capture sandwich” form...
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Published in | Clinical biochemistry Vol. 48; no. 9; pp. 603 - 608 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.06.2015
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Subjects | |
Online Access | Get full text |
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Summary: | This study established a novel time-resolved fluorescence immunoassay (TRFIA) that allows the simultaneous determination of rubella virus (RV) IgM and cytomegalovirus (CMV) IgM in human serum.
Lanthanum elements labeled antibody and streptavidin–biotin system were used in the “capture sandwich” format simultaneously.
The working range of TRFIA for RV IgM was 2–80AU/mL and for CMV IgM was 5–400AU/mL. Intra- and inter-assay coefficient of variation (CV) for RV IgM and CMV IgM were both less than 10% and recoveries were from 90% to 110%. No significant statistical difference in sensitivity or specificity was observed between dual-TRFIA and commercial chemiluminescent immunoassays (CLIA) in serum samples.
The novel dual-TRFIA for RV IgM and CMV IgM detection might have valuable clinical application, with satisfactory sensitivity, specificity and accuracy.
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•Provide a new approach for the quantitative detection of IgM antibodies•Develop a dual-TRFIA for RV and CMV IgM test simultaneously•No significant statistical difference between dual-TRFIA and commercial CLIA |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0009-9120 1873-2933 |
DOI: | 10.1016/j.clinbiochem.2015.01.009 |