Methodological approaches for histamine quantification using derivatization by chloroethylnitrosourea and ELISA measurement. Part II: Optimisation of the derivatization step
In a previous paper (part I), new strategy was used for raising antibodies against hapten (< 300 Da) and the quantification of these hapten by ELISA using derivatization by chloroethyl nitrosourea (CENU). After raising antibodies against histamine, they were characterized and used for ELISA measu...
Saved in:
Published in | Chemometrics and intelligent laboratory systems Vol. 80; no. 2; pp. 186 - 197 |
---|---|
Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
15.02.2006
Elsevier |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | In a previous paper (part I), new strategy was used for raising antibodies against hapten (<
300 Da) and the quantification of these hapten by ELISA using derivatization by chloroethyl nitrosourea (CENU). After raising antibodies against histamine, they were characterized and used for ELISA measurements. Optimal detection conditions were determined for the histamine quantification by ELISA method. The present study investigates the derivatization step of the histamine by chloroethylnitrosourea (CENU). Five factors (2 qualitative: nature of the solvent and nature of the antibodies and 3 quantitative: pH, % of solvent and time of derivatization) have been considered. Optimal reaction conditions were established by calculation of a validated model. |
---|---|
Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0169-7439 1873-3239 |
DOI: | 10.1016/j.chemolab.2005.06.007 |