Radish phospholipid hydroperoxide glutathione peroxidase provides protection against hydroperoxide-mediated injury in mouse 3T3 fibroblasts

• Published in: BMB reports Vol. 42; no. 10; pp. 648 - 654
• Main Authors: Li, Tian, Tsinghua University, Beijing, China, Liu, Guan-Lan, Tsinghua University, Beijing, China, Duan, Ming-Xing, Tsinghua University, Beijing, China, Liu, Jin-Yuan, Tsinghua University, Beijing, China
• Format: Journal Article
• Language: English
• Published: Korea (South) 31.10.2009
• Subjects: 3T3 Cells; Animals; Cell protection; Cell Survival - drug effects; Cytoprotection - drug effects; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - metabolism; Glutathione Peroxidase - pharmacology; Hydrogen Peroxide - toxicity; Hydroperoxide; Intracellular Space - drug effects; Intracellular Space - metabolism; Mice; Oxidative injury; Phospholipid hydroperoxide glutathione peroxidase; RABANO; RADIS; RADISHES; Raphanus - enzymology; Reactive Oxygen Species - metabolism; Recombinant Proteins - pharmacology
• ISSN: 1976-6696
• DOI: 10.5483/BMBRep.2009.42.10.648

Overexpression of phospholipid hydroperoxide glutathione peroxidase (PHGPx) genes has been reported to play an important role in protecting host cells from oxidative injury in several model systems. A radish phospholipid hydroperoxide glutathione peroxidase (RsPHGPx) known to have high catalytic activity was applied to mouse 3T3 fibroblasts to determine the protective effects of PHGPx against oxidative injury triggered by hydroperoxides such as hydrogen peroxide (H₂O₂), tert-butyl hydroperoxide (t-BHP) and phosphatidylcholine hydroperoxide (PCOOH). We observed that preincubation of cells with RsPHGPx significantly increased cell viability, reduced levels of malondialdehyde (MDA), inhibited generation of reactive oxygen species (ROS), and maintained natural cell shapes after treatment with H₂O₂, t-BHP or PCOOH, indicating that the exogenous RsPHGPx can act as an effective hydroperoxide-scavenger and may also protect target cells from oxidative damage. These results suggest the possibility for use of RsPHGPx as a therapeutic protectant.