Genomic changes and HPV type in cervical carcinoma

• Published in: Proceedings of the Society for Experimental Biology and Medicine Vol. 223; no. 3; p. 316
• Main Authors: Matthews, C P, Shera, K A, McDougall, J K
• Format: Journal Article
• Language: English
• Published: United States 01.03.2000
• Subjects: Chromosome Aberrations; Chromosome Mapping; Chromosomes, Human, Pair 3; DNA, Viral - analysis; Female; Humans; Loss of Heterozygosity; Nucleic Acid Hybridization; Papillomaviridae - genetics; Papillomaviridae - isolation & purification; Uterine Cervical Neoplasms - genetics; Uterine Cervical Neoplasms - pathology; Uterine Cervical Neoplasms - virology
• ISSN: 0037-9727
• DOI: 10.1046/j.1525-1373.2000.22345.x

To identify chromosomal regions that may include the loci of abnormally expressed cellular genes and may be specifically altered depending on the histological subtype of the tumor, we studied primary cervical carcinoma using CGH and HPV genotyping. Eighty-seven percent of the primary tumors were positive for DNA of a "high-risk" HPV type (e.g., 16 or 18). In the cervical carcinomas, without reference to histologic subtype, overrepresentation of chromosome 3q was the most consistent chromosomal aberration with underrepresentation of chromosome 3p also a frequent finding. Chromosome arms 1q, 5p, 20q, and Xq were overrepresented in many tumors and 3p loss and 5p, 8q, and 16q gain were only associated with squamous cell carcinoma in this series.