Isolation and characterization of novel Helicobacter spp. from the gastric mucosa of harp seals Phoca groenlandica

• Published in: Diseases of aquatic organisms Vol. 57; no. 1-2; pp. 1 - 9
• Main Authors: HARPER, Claudia G, SHILU XU, FOX, James G, ROGERS, Arlin B, YAN FENG, ZELI SHEN, TAYLOR, Nancy S, DEWHIRST, Floyd E, PASTER, Bruce J, MILLER, Melissa, HURLEY, Jenifer
• Format: Journal Article
• Language: English
• Published: Oldendorf Inter-Research 03.12.2003
• Subjects: Animal bacterial diseases; Animals; Bacterial diseases; Base Sequence; Biological and medical sciences; Cluster Analysis; DNA Primers; Gastric Mucosa - microbiology; Gastric Mucosa - pathology; Helicobacter; Helicobacter - cytology; Helicobacter - genetics; Helicobacter - isolation & purification; Helicobacter - ultrastructure; Helicobacter Infections - veterinary; Histological Techniques; Infectious diseases; Marine; Massachusetts; Medical sciences; Microscopy, Electron; Molecular Sequence Data; Phoca groenlandica; Phylogeny; Polymorphism, Restriction Fragment Length; RNA, Ribosomal - genetics; Seals, Earless - microbiology; Sequence Analysis, DNA; Species Specificity; Massachusetts; ANW, USA, Massachusetts; USA, Massachusetts; Protozoa; Cryptosporidium; Stomach; Apicomplexa; Carnivora; Spirillales; Helicobacter; Mucosa; Spirillaceae; Harp seal; Helicobacter infection; Pinnipedia; Gastritis; Infection; Phoca groenlandica; Vertebrata; Mammalia; Histopathology; Transmission electron microscopy; Ultrastructure; Digestive diseases; Bacteria; Pinniped
• ISSN: 0177-5103; 1616-1580
• DOI: 10.3354/dao057001

Since the recent discovery of Helicobacter cetorum in cetaceans and its role in the development of gastritis, speculation has existed as to whether pinnipeds have Helicobacter spp. associated gastritis and peptic ulcer disease. The gastric mucosa of 4 stranded harp seals Phoca groenlandica from the Massachusetts coastline were assessed for Helicobacter spp. by culture and PCR. We cultured 2 novel Helicobacter spp. from the pyloric antrum of 1 of the 4 harp seals studied, and identified these by PCR in 2 of the 4 seals. Both gram-negative bacterial isolates were catalase- and oxidase-positive. However, a fusiform helicobacter with flexispira morphology was urease-positive, and a spiral-shaped helicobacter was urease-negative. Slender, spiral and fusiform-shaped bacteria were detected in the gastric mucosa by the Warthin-Starry stain. Histopathologic analysis revealed mild diffuse lymphoplasmacytic gastritis within the superficial mucosa of the pyloric antrum of both infected seals. The 2 bacterial isolates were classified by 16S rRNA analysis; they clustered with other enteric helicobacters and represent 2 novel Helicobacter spp. The urease-negative bacterial isolate clustered with H. canis and the urease-positive isolate clustered with an isolate from a sea lion and isolates from sea otters. This cluster of pinniped isolates has 97 % similarity to a number of Helicobacter species, but appears to be most closely related to other helicobacters with flexispira morphology. These findings suggest that the novel Helicobacter spp. may play a role in the etiopathogenesis of gastrointestinal diseases in pinnipeds. To our knowledge, this represents the first isolation and characterization of a novel Helicobacter spp. from pinnipeds.