Fluorescence Determination of Azithromycin in Pharmaceutical Formulations by Using the Synchronous Scanning Approach After its Acid Derivatization

• Published in: Journal of fluorescence Vol. 23; no. 1; pp. 31 - 39
• Main Authors: Almeida, Vanessa G. K., Braga, Victor S. M., Pacheco, Wagner F., Cassella, Ricardo J.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 2013
• Subjects: Analytical Chemistry; Anti-Bacterial Agents - analysis; Anti-Bacterial Agents - chemistry; Azithromycin - analysis; Azithromycin - chemistry; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biomedicine; Biophysics; Biotechnology; Chemistry, Pharmaceutical; Fluorescence; Formulations; Hydrochloric Acid - chemistry; Kinetics; Limit of Detection; Original Paper; Pharmaceutical Preparations - chemistry; Pharmaceuticals; Reaction mechanisms; Reaction time; Scanning; Spectrometry, Fluorescence; Synchronous; Temperature; Pharmaceutical formulation; Antibiotic; Synchronous fluorescence; Azithromycin
• ISSN: 1053-0509; 1573-4994
• DOI: 10.1007/s10895-012-1111-8

In this present work, a fluorescence method for azithromycin (9-deoxo-9a-aza-9a-methyl-9a-homoerythromycin) determination in pharmaceutical formulations is proposed. The method is based on the synchronous fluorescence (Δλ = 30 nm, 482 nm) produced when azithromycin is derivatized in strong acidic medium (9.0 mol L −1 HCl). The influence of the derivatization conditions (acid concentration, reaction time and temperature) was studied. Also, the possible reaction mechanism was discussed. In the optimized conditions, the method presented a limit of detection of 0.23 mg L −1 and a limit of quantification of 0.76 mg L −1 . The developed procedure was successfully applied in the determination of azithromycin in pharmaceutical formulations.