Revision in the first steps of the biosynthesis of the red antibiotic prodigiosin: use of a synthetic thioester to validate a new intermediate

A biosynthetic pathway for the red-antibiotic, prodigiosin, was proposed over a decade ago but not all the suggested intermediates could be detected experimentally. Here we show that a thioester that was not originally included in the pathway is an intermediate. In addition, the enzyme PigE was orig...

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Published inRSC chemical biology Vol. 2; no. 2; pp. 551 - 555
Main Authors Couturier, Maxime, Bhalara, Hiral D, Monson, Rita E, Salmond, George P. C, Leeper, Finian J
Format Journal Article
LanguageEnglish
Published RSC 01.04.2021
Subjects
Chemistry
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Summary:A biosynthetic pathway for the red-antibiotic, prodigiosin, was proposed over a decade ago but not all the suggested intermediates could be detected experimentally. Here we show that a thioester that was not originally included in the pathway is an intermediate. In addition, the enzyme PigE was originally described as a transaminase but we present evidence that it also catalyses the reduction of the thioester intermediate to its aldehyde substrate. A revision is proposed to the biosynthetic pathway to the well-known red pigment prodigiosin via a new thioester intermediate.
Bibliography:10.1039/d0cb00173b
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ISSN:2633-0679
2633-0679
DOI:10.1039/d0cb00173b