Study of the radiotherapy sensitization effects and mechanism of capecitabine (Xeloda) against non-small-cell lung cancer cell line A549

• Published in: Genetics and molecular research Vol. 14; no. 4; pp. 16386 - 16391
• Main Authors: Zhu, J J, Shan, J J, Sun, L B, Qiu, W S
• Format: Journal Article
• Language: English
• Published: Brazil 09.12.2015
• Subjects: Antimetabolites, Antineoplastic - pharmacology; Apoptosis - drug effects; Apoptosis - radiation effects; Capecitabine - pharmacology; Carcinoma, Non-Small-Cell Lung; Cell Cycle Checkpoints - drug effects; Cell Cycle Checkpoints - radiation effects; Cell Line, Tumor; Cell Proliferation - drug effects; Cell Proliferation - radiation effects; Humans; Lung Neoplasms; Radiation Tolerance - drug effects; Radiation, Ionizing
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/2015.December.9.7

The purpose of this study was to explore the radiotherapy sensitization effects and the mechanism of capecitabine (Xeloda) against the non-small-cell lung cancer cell line, A549. γ-[(60)Co] radiation was used as the intervention method. Proliferative inhibition of capecitabine on A549 cells was determined by the CCK-8 method. The effects of capecitabine on the apoptosis rate and cell cycle distribution of A549 were detected with the flow cytometric method. We found that capecitabine inhibited the proliferation of A549 in a dose-dependent manner, notably increased the cell apoptosis rate and blocked the cellular G0/G1 phase after radiotherapy by γ-[(60)Co]. Therefore, capecitabine can significantly increase the radiosensitivity of A549; its mechanism may be related to cell cycle arrest and induction of apoptosis.