Methionine Transport in Chlorella vulgaris

Absorption of 35S-L-methionine by Chlorella vulgaris was measured at concentrations that ranged from 0.1 to 10.0 μmoles/ml. A brief, rapid phase of uptake was followed by a more prolonged, slower phase that was linear only at the lowest concentrations. The radioactivity accumulated by the end of 1 h...

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Bibliographic Details
Published inPlant physiology (Bethesda) Vol. 41; no. 3; pp. 405 - 410
Main Author Shrift, Alex
Format Journal Article
LanguageEnglish
Published United States American Society of Plant Physiologists 01.03.1966
Subjects
Active transport
Amino acids
Azides
Biological Transport, Active
Eukaryota - metabolism
Hematocrit
In Vitro Techniques
Methionine - metabolism
Molecules
Paper chromatography
Plants
Radioactive decay
Sulfates
Sulfur
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Summary:Absorption of 35S-L-methionine by Chlorella vulgaris was measured at concentrations that ranged from 0.1 to 10.0 μmoles/ml. A brief, rapid phase of uptake was followed by a more prolonged, slower phase that was linear only at the lowest concentrations. The radioactivity accumulated by the end of 1 hour's incubation at an exogenous level of 0.1 μmole/ml was retained by the cells despite the inclusion of 10 μmoles/ml of nonradioactive methionine in the rinse medium. As the exogenous concentration was raised, the ratio of intracellular soluble radioactivity to exogenous radioactivity decreased. Analysis of the accumulated, soluble radioactivity showed that 90% was in the form of methionine and that about 10% had been converted to a compound with properties of S-adenosylmethionine. Azide and ethionine were the most effective of the inhibitors tested.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.41.3.405