Early and Late Effects of Low-Dose X-ray Exposure in Human Fibroblasts: DNA Repair Foci, Proliferation, Autophagy, and Senescence

• Published in: International journal of molecular sciences Vol. 25; no. 15; p. 8253
• Main Authors: Osipov, Andrey, Chigasova, Anna, Yashkina, Elizaveta, Ignatov, Maxim, Vorobyeva, Natalia, Zyuzikov, Nikolay, Osipov, Andreyan N
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.08.2024
• Subjects: Autophagy; Autophagy - radiation effects; Cancer; Cell growth; Cell Proliferation - radiation effects; Cells, Cultured; Cellular Senescence - radiation effects; DNA; DNA damage; DNA Damage - radiation effects; DNA damage response; DNA double-strand breaks; DNA repair; DNA Repair - radiation effects; DNA repair foci; Dose-Response Relationship, Radiation; Fibroblasts; Fibroblasts - metabolism; Fibroblasts - radiation effects; Histones - metabolism; Humans; Kinases; proliferation; Proteins; Radiation; Scientific equipment and supplies industry; Senescence; Tumor proteins; Tumor Suppressor p53-Binding Protein 1 - metabolism; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism; X-Rays - adverse effects; Yeast; United States; Massachusetts; Russia; senescence; fibroblasts; autophagy; proliferation; DNA damage response; low-dose effects; X-ray radiation; DNA repair foci; DNA double-strand breaks
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25158253

The effects of low-dose radiation exposure remain a controversial topic in radiation biology. This study compares early (0.5, 4, 24, 48, and 72 h) and late (5, 10, and 15 cell passages) post-irradiation changes in γH2AX, 53BP1, pATM, and p-p53 (Ser-15) foci, proliferation, autophagy, and senescence in primary fibroblasts exposed to 100 and 2000 mGy X-ray radiation. The results show that exposure to 100 mGy significantly increased γH2AX, 53BP1, and pATM foci only at 0.5 and 4 h post irradiation. There were no changes in p-p53 (Ser-15) foci, proliferation, autophagy, or senescence up to 15 passages post irradiation at the low dose.