White spot syndrome virus (WSSV) detected by PCR in rotifers and rotifer resting eggs from shrimp pond sediments

• Published in: Diseases of aquatic organisms Vol. 59; no. 1; pp. 69 - 73
• Main Authors: YAN, Dong-Chun, DONG, Shuang-Lin, JIE HUANG, YU, Xiao-Ming, FENG, Min-Yi, LIU, Xiang-Yi
• Format: Journal Article
• Language: English
• Published: Oldendorf Inter-Research 21.04.2004
• Subjects: Animal aquaculture; Animal productions; Animals; Aquaculture; Biological and medical sciences; Crustacea; DNA Primers; DNA Viruses - genetics; Fenneropenaeus chinensis; Fundamental and applied biological sciences. Psychology; Invertebrate aquaculture; Marine; Molecular Probe Techniques; Ovum - virology; Penaeidae; Polymerase Chain Reaction; Rotifera; Rotifera - virology; White spot syndrome virus; Overwintering; Reservoir; Epizootics; Macrura; Crustacea; Marine environment; Breeding pond; Transmission from animal to animal; Decapoda; Rotifera; White spot syndrome virus; Egg; Transmission route; Zoopathogen; Sediments; Infection; Polymerase chain reaction; Virus; Edible crustacean; Penaeus chinensis; Arthropoda; Viral disease; Rotifer resting eggs; Invertebrata; Detection; Aquaculture
• ISSN: 0177-5103; 1616-1580
• DOI: 10.3354/dao059069

White spot syndrome virus (WSSV) was detected by PCR-dot blot hybridization in rotifer resting eggs from shrimp Penaeus chinensis culture-pond sediments. It was also detected in rotifers hatched from those eggs. Surface disinfection before analysis indicated that WSSV was probably present within the resting eggs. Results suggested that rotifer resting eggs may be an overwintering reservoir for WSSV in shrimp ponds.