Knee replacement surgery significantly elevates the urinary inflammatory biomarkers neopterin and 7,8-dihydroneopterin

• Published in: Clinical biochemistry Vol. 63; pp. 39 - 45
• Main Authors: Baxter-Parker, Gregory, Roffe, Lloyd, Cross, Sean, Frampton, Chris, Hooper, Gary J., Gieseg, Steven P.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2019
• Subjects: 7,8-dihydroneopterin; 7,8-dihydroneopterin stability; Adult; Aged; Arthroplasty, Replacement, Knee; Biomarkers - urine; Female; Humans; Inflammation; Inflammation - urine; Knee-arthroplasty; Male; Middle Aged; Neopterin; Neopterin - analogs & derivatives; Neopterin - urine; Oxidative stress; Preservation, Biological - methods; Specimen Handling - methods; Surgical stress, trauma; Surgical stress, trauma; Knee-arthroplasty; Oxidative stress; 7,8-dihydroneopterin stability; 7,8-dihydroneopterin; Inflammation; Neopterin
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/j.clinbiochem.2018.11.002

Knee arthroplasty surgery is significant trauma, leading to an activated immune system causing inflammation and oxidative stress. Many current biomarkers are invasive, costly, and often slow to analyse, limiting their use for rapid inflammatory measurements. We have examined the use of urinary neopterin and total neopterin in knee arthroplasty patients to non-invasively measure oxidative stress and inflammation from immune system activation. We aim to validate the use of these biomarkers for quick, cost effective and predictive measurements of post-surgical inflammation assessment. 19 Knee arthroplasty patients were analysed pre-operatively and for a defined post-operative period to determine the urinary levels of neopterin and total neopterin (neopterin +7,8-dihydroneopterin) using high performance liquid chromatography with fluorescence detection. These results were then compared to a control group of 20 participants with normal knee function. 7,8-Dihydroneopterin was stable in urine over 12 h when refrigerated. Knee arthritis was associated with an increase in pre-operative neopterin (oxidative stress) and total neopterin (inflammation). The subsequent arthroplasty surgery generated a significant increase neopterin and total neopterin. Both biomarkers were reduced immediately post-operatively, before becoming elevated on the following days. There was no clear evidence of an association between initial neopterin and total neopterin levels and a patient's level of inflammation during in-hospital recovery. The stability of 7,8-dihydroneopterin in urine allows for its use as an inflammatory marker. Urinary neopterin and total neopterin provided a fast, non-invasive, and simple measure of oxidative stress and inflammation after knee arthroplasty. •Urinary 7,8-dihydroneopterin is stable over 12 h when refrigerated at 4 °C, allowing for reliable analysis of total neopterin.•Neopterin and total neopterin are elevated in response to knee arthroplasty surgery so providing a rapid, non-invasive, marker of oxidative stress and inflammation.•Obtaining urine is convenient and non-invasive, allowing for easier analysis than blood derived biomarkers during patient recovery.