A comprehensive in vitro investigation of a portable magnetic separator device for human blood detoxification

• Published in: Physics in medicine & biology Vol. 52; no. 19; pp. 6053 - 6072
• Main Authors: Chen, Haitao, Ebner, Armin D, Bockenfeld, Danny, Ritter, James A, Kaminski, Michael D, Liu, Xianqiao, Rempfer, Dietmar, Rosengart, Axel J
• Format: Journal Article
• Language: English
• Published: England IOP Publishing 07.10.2007
• Subjects: Blood Chemical Analysis; Computer Simulation; Computer-Aided Design; Equipment Design; Equipment Failure Analysis; Flow Injection Analysis - instrumentation; Flow Injection Analysis - methods; Hemofiltration - instrumentation; Hemofiltration - methods; Humans; Immunomagnetic Separation - instrumentation; Immunomagnetic Separation - methods; Magnetics - instrumentation; Magnetics - therapeutic use; Models, Cardiovascular
• ISSN: 0031-9155; 1361-6560
• DOI: 10.1088/0031-9155/52/19/023

A portable magnetic separator device is being developed for a proposed magnetically based detoxification system. In this paper, the performance of this device was evaluated via preliminary in vitro flow experiments using simple fluids and a separator unit consisting of one tube and two metal wires, each at the top and bottom of the tube. The effects of the following factors were observed: mean flow velocity U(o) (0.14-45 cm s(-1)), magnetic field strength micro(o)H(o) (0.125-0.50 T), wire size R(w) (0.125, 0.250 and 0.500 mm), wire length L(w) (2, 5 and 10 cm), wire materials (nickel, stainless steel 304 and 430) and tube size (outer radius R(o) = 0.30 mm and inner radius R(i) = 0.25 mm; R(o) = 0.50 mm and R(i) = 0.375 mm; and R(o) = 2.0 mm and R(i) = 1.0 mm). Our observations showed that the experimental results fit well with the corresponding theoretical results from the model we previously developed at a low flow velocity area (for example, U(o) < or = 20 cm s(-1)), strong external magnetic field (for example, > or = 0.30 T) and long wire length (for example, L(w) = 10 cm). The experimental results also showed that more than 90% capture efficiency is indeed achievable under moderate systemic and operational conditions. Pressure drop measurements revealed that the device could work well under human physiological and clinical conditions, and sphere buildup would not have any considerable effect on the pressure drop of the device. The breakthrough experiments demonstrated that a lower flow rate V, higher applied magnetic field micro(o)H(o) and diluted sphere suspension, i.e. lower C(o), would delay the breakthrough. All the results indicate the promise of this portable magnetic separator device to efficiently in vivo sequestrate nano-/micro-spheres from blood flow in the future magnetically based detoxification system.