Caspase 3, 6, 8, and 9 Gene Expression in Peripheral Blood Leukocytes and Plasma Concentrations of IL-6 and TNF-α in Carriers of Different Polymorphic Marker -174G>C Genotypes of IL6 Gene Associated with the Risk of Nonalcoholic Steatohepatitis

• Published in: Bulletin of experimental biology and medicine Vol. 162; no. 3; pp. 370 - 374
• Main Authors: Kurbatova, I. V., Topchieva, L. V., Dudanova, O. P.
• Format: Journal Article
• Language: English
• Published: New York Springer US 2017
• Subjects: Adult; Alleles; Biomedical and Life Sciences; Biomedicine; Caspase 3 - genetics; Caspase 3 - immunology; Caspase 6 - genetics; Caspase 6 - immunology; Caspase 8 - genetics; Caspase 8 - immunology; Caspase 9 - genetics; Caspase 9 - immunology; Cell Biology; Female; Gene Expression Regulation; Gene Frequency; Genetic Predisposition to Disease; Heterozygote; Humans; Interleukin-6 - genetics; Interleukin-6 - immunology; Internal Medicine; Laboratory Medicine; Leukocytes, Mononuclear - immunology; Leukocytes, Mononuclear - metabolism; Leukocytes, Mononuclear - pathology; Male; Middle Aged; Non-alcoholic Fatty Liver Disease - diagnosis; Non-alcoholic Fatty Liver Disease - epidemiology; Non-alcoholic Fatty Liver Disease - genetics; Non-alcoholic Fatty Liver Disease - immunology; Pathology; Polymorphism, Single Nucleotide; Risk Factors; Russia - epidemiology; Signal Transduction; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - immunology; nonalcoholic steatohepatitis; polymorphism; caspases; interleukin-6
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-017-3618-0

We revealed an association of IL6 gene - 174G > C polymorphism with the development of nonalcoholic steatohepatitis in the Russian population. The risk is significantly higher in carriers of C allele: OR=1.77 (1.04; 3.02). The effects of - 174G > C substitution in IL6 gene involving caspase 9 gene transcripts in peripheral blood leukocytes and on blood content of TNF-α in healthy individuals without clinical manifestations of nonalcoholic steatohepatitis were detected. The content of caspase 9 gene transcripts in peripheral blood leukocytes and plasma level of TNF-α were significantly higher in healthy subjects carrying C allele than in carriers of GG genotype. The levels of caspases 3, 6, 8, and 9 gene transcripts in peripheral blood leukocytes and plasma concentrations of TNF-α in patients with nonalcoholic steatohepatitis did not depend on IL6 genotype by - 174G < C marker.