Fine mapping of a fertility restoring gene for a new CMS hybrid rice system

• Published in: Molecular breeding Vol. 36; no. 10; pp. 1 - 10
• Main Authors: Li, Yu, Zhang, Min, Yang, Xiaofei, Lin, Chunfang, Duan, Yuanlin, Wang, Naiyuan
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.10.2016; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biotechnology; Chromosome 10; Chromosomes; Cloning; Cytoplasm; Cytoplasmic male sterility; Fertility; Gene mapping; Gene polymorphism; Genetic analysis; Hybrid systems; Life Sciences; Linkage analysis; Male sterility; Mapping; Molecular biology; Nuclei; Oryza rufipogon; Oryza sativa; Oryza sativa indica; Plant biology; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Polymorphism; Restoration; Rice; Fine mapping; Fertility restoration gene; Linkage genetic analysis; CMS-FA; Rice
• ISSN: 1380-3743; 1572-9788
• DOI: 10.1007/s11032-016-0561-0

The Fujian Abortion cytoplasmic male sterility (CMS-FA) system, a new type of sporophytic CMS system in indica rice ( Oryza sativa L.), was developed using the cytoplasm and the corresponding fertility-restoring gene from a wild rice ( O. rufipogon L.), which originated from Fujian Province, China. Previous studies in combination with several years of production practice demonstrated that CMS-FA hybrid rice was superior to CMS-WA hybrid rice, a prevailing hybrid rice worldwide, and that the male fertility restoration was controlled by a pair of dominant alleles. We tentatively designated the fertility restoration gene as Rf(fa) . The analysis of the polymorphism between the fertile and sterile pool DNAs from a mapping segregation population (BC1F1) indicated that Rf(fa) was located on rice chromosome 10. We further delimited the Rf(fa) locus to a 121.1-kb region flanked by RM6100 and MM2023, which were approximately 0.26 cM and 0.18 cM away from Rf(fa) , respectively, by simple sequence repeat molecular marker linkage genetic analysis. These results would facilitate the map-based cloning of Rf(fa) , the elucidation of a novel molecular mechanism underlying cytoplasm–nucleus interaction in the CMS-FA system, and the production application of this hybrid rice.