Induction of abortion with aglepristone significantly changed the expression of progesterone and estrogen receptors in canine endometrial stromal cells

• Published in: Theriogenology Vol. 70; no. 9; pp. 1439 - 1448
• Main Authors: Kanca, H., Walter, I., Schäfer-Somi, S., Budik, S., Ay, S.S., Kucukaslan, I., Agaoglu, A.R., Izgur, H., Aslan, S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2008; [Oxford]: Butterworth-Heinemann; [New York]: Elsevier Science
• Subjects: Abortifacient Agents - pharmacology; abortifacients; abortion (animals); Abortion, Induced - veterinary; Abortion, Veterinary - chemically induced; Aglepristone; Animals; Bitch; Dogs; Endometrium; Endometrium - cytology; Endometrium - metabolism; Estradiol - blood; Estrenes - pharmacology; Estrogen receptor; estrogens; Female; gene expression; gene expression regulation; Gene Expression Regulation - drug effects; hormone receptors; immunohistochemistry; induced abortion; myometrium; placenta; Placenta - metabolism; Pregnancy; Pregnancy termination; progesterone; Progesterone - blood; Progesterone receptor; progesterone receptors; Receptors, Estrogen - genetics; Receptors, Estrogen - metabolism; Receptors, Progesterone - genetics; Receptors, Progesterone - metabolism; Reverse Transcriptase Polymerase Chain Reaction - veterinary; Stromal Cells - cytology; Stromal Cells - metabolism; Aglepristone; Bitch; Estrogen receptor; Progesterone receptor; Endometrium; Pregnancy termination
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2008.04.045

In the present study, resorption/abortion was induced between days 25 and 45 of gestation with aglepristone (group IRA, n = 10). The aim was to observe the change in the distribution of progesterone (PR) and estrogen receptors (ER), in comparison to a group of spontaneous resorptions/abortions (group SRA, n = 5), and a further group of normal healthy pregnant animals, ovariohysterectomized between days 25 and 45 of gestation (controls, n = 7). The receptors were assessed by means of immunohistochemistry (IHC) and RT-PCR, at the placental and interplacental sites of the uterine horn as well as in the corpus uteri. Significant differences were observed between the controls on one side and the groups of resorption/abortion on the other side. The total scores of the progesterone receptors (TPR) in the placental and interplacental part of the uterine horn, was significantly lower in the endometrial stromal cells (ESC) of the control group than in those of the SRA- and IRA-group, respectively (placenta: 5.8 vs. 6.5 and 6.7, p < 0.01; interplacental sites: 5.6 vs. 6.6 and 6.6, p < 0.05). In contrary, the total scores of the estrogen receptors (TER) at interplacental sites and the corpus uteri, respectively, was significantly higher in the myometrial smooth muscle cells (MSMC) and the ESC ( p < 0.05) of the controls. We therefore conclude, that the here observed differences between groups point to an up-regulation of TPR- and a down-regulation of TER-scores in endometrial stromal cells at different uterine sites during resorption/abortion, which indicates a special role of these cells.