Phagocytic Cells as Effectors in a Cell-Mediated Immunity System

Abstract Cell-mediated immunity, using a xenogeneic system, i.e., mouse spleen cells attacking chicken erythrocytes (CRBC) is studied. In this system target cell lysis by spleen cells from immunized animals is complement independent, hence cell-mediated. It is specific because trinitrophenyl-(TNP) s...

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Published inThe Journal of immunology (1950) Vol. 111; no. 6; pp. 1844 - 1854
Main Authors Dennert, Gunther, Lennox, Edwin S
Format Journal Article
LanguageEnglish
Published United States Am Assoc Immnol 01.12.1973
Subjects
Animals
Antilymphocyte Serum
Cell Separation
Cells, Cultured
Chickens
Chromium Radioisotopes
Complement System Proteins
Cytotoxicity Tests, Immunologic
Immunity, Cellular
Iron
Lymphocytes - immunology
Magnetics
Mice
Mice, Inbred AKR - immunology
Phagocytosis
Radiation Chimera
Spleen - cytology
T-Lymphocytes - immunology
Thymectomy
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Summary:Abstract Cell-mediated immunity, using a xenogeneic system, i.e., mouse spleen cells attacking chicken erythrocytes (CRBC) is studied. In this system target cell lysis by spleen cells from immunized animals is complement independent, hence cell-mediated. It is specific because trinitrophenyl-(TNP) sensitized spleens lyse TNP-CRBC but not CRBC. CRBC-sensitized thymus-derived (T) lymphocytes cooperate with bone marrow-derived lymphocytes in induction of CRBC plaque-forming cells but cannot lyse CRBC as target. However, anti-CRBC antibody at concentrations too low to be detected by complement dependent lysis do induce cytotoxicity to CRBC in spleen cell suspensions containing T cells as well as in spleen cell suspensions depleted of T cells. Anti-ϑ serum absorbed to remove non-anti-ϑ antibody does not abrogate the cytotoxicity of immune spleen cells. These results indicate that the effector cell in this system is not a ϑ-antigen-bearing T lymphocyte. The effector cell is abundant in spleen and in the peritoneal cavity but not in lymph nodes of normal animals: it is bone marrow-derived, radiation resistant, and sticks to siliconized glass bead columns. Since, in addition, the effector cell can be separated from other spleen cells by treatment with carbonyl iron powder and a magnet, it is, we suggest, a phagocytic cell.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.111.6.1844