Simple, Rapid Determination of Lecithin and Sphingomyelin in Amniotic Fluid

Abstract We describe a rapid and very sensitive modified determination of lecithin and sphingomyelin in amniotic fluid. It requires 1.5 ml of amniotic fluid and can be done in less than 40 min. Modifications include "Instant Thin Layer Chromatography" plates (Gelman) with a punched disc sy...

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Bibliographic Details
Published inClinical chemistry (Baltimore, Md.) Vol. 19; no. 12; pp. 1394 - 1396
Main Authors Blass, K. G, Thibert, R. J, Draisey, T. F
Format Journal Article
LanguageEnglish
Published England Am Assoc Clin Chem 01.12.1973
Subjects
Amniotic Fluid - analysis
Chromatography, Thin Layer
Coloring Agents
Evaluation Studies as Topic
Female
Humans
Infant, Newborn
Methods
Phosphatidylcholines - analysis
Pregnancy
Pregnancy Complications
Respiratory Distress Syndrome, Newborn - diagnosis
Sphingomyelins - analysis
Ultraviolet Rays
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Summary:Abstract We describe a rapid and very sensitive modified determination of lecithin and sphingomyelin in amniotic fluid. It requires 1.5 ml of amniotic fluid and can be done in less than 40 min. Modifications include "Instant Thin Layer Chromatography" plates (Gelman) with a punched disc system of sample application, as well as elimination of the acetone wash steps, and substitution of Rhodamine B dye for the corrosive sulfuric acid char technique of detection. The procedure can be quantified, and exact concentrations of lecithin in amniotic fluid can be determined by a standard addition procedure, or from a standard curve.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/19.12.1394