The taxonomy and growth of a Crypthecodinium species (Dinophyceae) isolated from a brackish-water fish aquarium

An unidentified heterotrophic dinoflagellate found growing in abundance in a brackish-water fish aquarium was isolated and serially cultivated using a fish cell line as the food source. Prominent characteristics of this dinoflagellate included a cingulum that did not fully encircle the motile cell,...

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Bibliographic Details
Published inAfrican journal of marine science Vol. 28; no. 2; pp. 185 - 191
Main Authors Parrow, MW, Elbrächter, M, Krause, MK, Burkholder, JM, Deamer, NJ, Htyte, N, Allen, EH
Format Journal Article
LanguageEnglish
Published Taylor & Francis Group 01.09.2006
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Summary:An unidentified heterotrophic dinoflagellate found growing in abundance in a brackish-water fish aquarium was isolated and serially cultivated using a fish cell line as the food source. Prominent characteristics of this dinoflagellate included a cingulum that did not fully encircle the motile cell, cell division in non-motile cysts, and a theca composed of thin but structured plates. Morphological analysis of flagellate cells by scanning electron microscopy revealed a Kofoid thecal plate tabulation of 4', 4a, 4", 'X', 5 or 6c, ?s, 5"', 1p, 1"", most consistent with the original description of Crypthecodinium setense Biecheler. This Crypthecodinium species exhibited a high maximum division rate (3.2 divisions day −1 ) and cell yield (>10 6 cells ml −1 ) when fed cultured fish cells. Small sub-unit rDNA phylogenetic analyses supported relatedness with a previously studied Crypthecodinium-like dinoflagellate, but a significant difference in aligned gene sequences was found. This study provides the first clear demonstration of the plate tabulation of a Crypthecodinium species since the original description over 60 years ago, allowing the original morphological conception of Crypthecodinium to be linked with molecular phylogenetic information.
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ISSN:1814-232X
1814-2338
DOI:10.2989/18142320609504145