The taxonomy and growth of a Crypthecodinium species (Dinophyceae) isolated from a brackish-water fish aquarium
An unidentified heterotrophic dinoflagellate found growing in abundance in a brackish-water fish aquarium was isolated and serially cultivated using a fish cell line as the food source. Prominent characteristics of this dinoflagellate included a cingulum that did not fully encircle the motile cell,...
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Published in | African journal of marine science Vol. 28; no. 2; pp. 185 - 191 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Taylor & Francis Group
01.09.2006
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Subjects | |
Online Access | Get full text |
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Summary: | An unidentified heterotrophic dinoflagellate found growing in abundance in a brackish-water fish aquarium was isolated and serially cultivated using a fish cell line as the food source. Prominent characteristics of this dinoflagellate included a cingulum that did not fully encircle the motile cell, cell division in non-motile cysts, and a theca composed of thin but structured plates. Morphological analysis of flagellate cells by scanning electron microscopy revealed a Kofoid thecal plate tabulation of 4', 4a, 4", 'X', 5 or 6c, ?s, 5"', 1p, 1"", most consistent with the original description of Crypthecodinium setense Biecheler. This Crypthecodinium species exhibited a high maximum division rate (3.2 divisions day
−1
) and cell yield (>10
6
cells ml
−1
) when fed cultured fish cells. Small sub-unit rDNA phylogenetic analyses supported relatedness with a previously studied Crypthecodinium-like dinoflagellate, but a significant difference in aligned gene sequences was found. This study provides the first clear demonstration of the plate tabulation of a Crypthecodinium species since the original description over 60 years ago, allowing the original morphological conception of Crypthecodinium to be linked with molecular phylogenetic information. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1814-232X 1814-2338 |
DOI: | 10.2989/18142320609504145 |