Time-resolved nonlinear fluorescence spectroscopy using femtosecond multiphoton excitation and single-photon timing detection

• Published in: Measurement science & technology Vol. 8; no. 11; pp. 1339 - 1349
• Main Authors: Volkmer, Andreas, Hatrick, David A, Birch, David J S
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.11.1997; Institute of Physics
• Subjects: Exact sciences and technology; Infrared, submillimeter wave, microwave and radiowave instruments, equipment and techniques; Instruments, apparatus, components and techniques common to several branches of physics and astronomy; Physics; Time-resolved optical spectroscopies and other ultrafast optical measurements in condensed matter; Fluorescence spectroscopy; Time resolution; Lifetime; Phenyl compounds; Multiphoton excitation; Hydrocarbons; Measuring methods; fs range; Correlation time; Molecular probe; Experimental study; Xanthene dye
• ISSN: 0957-0233; 1361-6501
• DOI: 10.1088/0957-0233/8/11/021

Using a femtosecond Ti:sapphire laser system, the authors have observed two photon induced fluorescence from 1,6-diphenylhexatriene and rhodamine 6G and three photon induced fluorescence from \+i\p\-i\-terphenyl in propylene glycol. A single photon timing nonlinear fluorometer recorded the fluorescence decay times and rotational correlation times of molecular probes. A microchannel plate photomultiplier giving 70 ps impulse response was used for detection. The results reveal the potential of multiphoton induced fluorescence anisotropy in the study of microheterogeneous systems (biomembranes, porous matrices, etc) by selecting the excitation wavelength and class of probe molecule. (Original abstract - amended)